摘要
Germination could improve the sensory and nutritional value of brown rice(BR),and magnetic field(MF)is considered as a promising technique for enhancing germination.However,the mechanism underlying MFenhanced germination of BR remains unclear.Therefore,in this study,BR was exposed to MF(10 mT,60 min,25℃),germinated in the absence/presence of gibberellin(GA)solution(20 mg/L,30℃)for 36 h,and subsequently the mechanism was explored by assessing germination index,endogenous GA content,relevant gene expression,α-amylase activity,and α-amylase action on rice starch.Results indicated that with MF treatment,the shoot and root length of BR increased by 10.4% and 22.9%,respectively.MF treatment upregulated the gene expression related toα-amylase by 0-110.2%,activatedα-amylase by 11.0%,led to the hydrolysis of starch into more reducing sugars by 9.2% and thus provided additional energy for BR germination.Post-germination,pores appeared on BR starch surface,and there was a decrease in its relative crystallinity,pasting temperature,and enthalpy.Compared with MF,the combination of MF and GA treatments(GM)showed a more pronounced effect on germination,which further confirmed that MF might enhance BR germination by activating α-amylase through the GA pathway.Overall,this study extended the knowledge of MF-assisted germination,which provided a guidance for the applications of MF in food and agricultural industries.
基金
financially supported by National Nature Science Foundation of China(32102131)
China Postdoctoral Science Foundation(2020M681631).
