摘要
A Multi species Probiotics(MSP)of calf gut origin was encapsulated either with chitosan(ECMSP)or without chitosan(EMSP).Further,the probiotic was encapsulated and lyophilized(ELMSP)using three types of cryoprotectant solutions sucrose(ELMSP(S)),skim milk(ELMSP(SM))and sucrose+skim milk combination(ELMSP(SSM)).The encapsulation yield was good and ranged from 75% to 80% for MSP products.These MSP products were stored at refrigeration temperature(4℃),deep freeze and room temperature for 12 weeks.The shelf life(mean CFU)of encapsulated microcapsules was significantly better(P<0.001)at the refrigeration temperature(9.44 Log10 CFU/g)than deep freeze(7.42 Log10 CFU/g)and room temperature(6.67 Log10 CFU/g).At the end of the 12 weeks storage period,EMSP maintained 25.1%,49.6% and 32.6% viable counts at room temperature,refrigeration and deep freeze condition respectively.For the lyophilized-encapsulated MSP,the mean probiotic counts of ELMSP(S)and ELMSP(SSM)were significantly(P<0.001)higher(9.62 Log10 CFU/g)than those of ELMSP(SM)((9.36 Log10 CFU/g))at room temperature.At refrigeration temperature,the mean probiotic counts for ELMSP(SSM.(10.1 Log10 CFU/g))were significantly higher(P<0.001)than ELMSP(S,(9.97 Log10 CFU/g))and ELMSP(SM,(9.86 Log10 CFU/g)).Further,a 12 weeks storage study for ELMSP(SSM)showed that the microcapsules stored at refrigeration temperature(4℃)showed only 6.4% drop in viability which was significantly lower(P<0.001)than that of room temperature showing 15.3% drop and deep freeze storage with 17.1%drop in viability.It was appreciable that even at room temperature;there was only one log drop in the viability of MSP showing that it can be comfortably stored at room temperature for longer periods at field level.
基金
ICAR-VTCC for providing the funding
Director,ICAR-IVRI for providing the infrastructural support.
