摘要
Ophiocordyceps sinensis is a prized edible and medicinal fungus due to its high nutritional value and broad pharmacological properties.The quality of O.sinensis from Nagqu in Xizang has been regarded as superior to those from other regions.However,the mechanisms underlying these differences remain unclear.This study investigated the metabolic profiles and gene expression patterns of O.sinensis from five major production areas on the Tibetan Plateau in China using metabolomics and transcriptomics.A total of 349 differentially accumulated metabolites(DAMs)and 2983 differentially expressed genes(DEGs)were identified.O.sinensis from Nagqu was characterized by 42 DAMs,primarily including amino acids,nucleosides,fatty acids,and vitamins.Integrated metabolomic and transcriptomic data indicated that DAMs and DEGs were co-enriched in pathways associated with amino acid metabolism,carbohydrate metabolism,lipid metabolism,and nucleotide metabolism.Additionally,the genes ADE5,pgm,purC,IMPDH,ADSS,AK,CarA,CarB,and OMPD were suggested to play critical roles in the biosynthesis of nucleosides,while the genes LSS,CYP51-1,and ERG25 were implicated in steroid biosynthesis.These findings enhance our understanding of the regulatory mechanisms of bioactive compound synthesis in O.sinensis.
基金
supported by grants from the National Natural Sciences Foundation of China(No.82060779)
the Guizhou Provincial Basic Research Program(Natural Science,No.ZK[2022]Y323).
