摘要
In this work,different ratios of α/β-Cyclodextrin and carbonyldiimidazole as a cross linker were employed for formulation of α/β-Cyclodextrin Nanosponges wherein 1:6 M ratio was finalized for preparation of Cinnamon Extract/trans-Cinnamaldehyde loaded Cyclodextrin Nanosponges via freeze drying.β-Cyclodextrin Nanosponges were found to have greater encapsulation efficiency for trans-Cinnamaldehyde(93.30%±1.94%)and Cinnamon Extract(35.64%±2.08%)compared to α-Cyclodextrin Nanosponges.FTIR spectra of loaded Cyclodextrin Nanosponges did not display any representative peaks of the Cinnamon Extract/trans-Cinnamaldehyde,confirming inclusion complexation.X-ray Diffraction and Differential Scanning Calorimetry results disclosed the amorphous nature of blank and loaded Cyclodextrin Nanosponges.Cinnamon Extract loadedα/β-Cyclodextrin Nanosponges displayed relatively smaller particle size(447-580 nm)compared to trans-Cinnamaldehyde loaded α/β-Cyclodextrin Nanosponges(630-651 nm).SEM illustrated the porous nature of Cyclodextrin Nanosponges,while TEM depicted the spherical outline of Cinnamon extract-loaded-β-Cyclodextrin Nanosponges with an average diameter of 495 nm.Cinnamon Extract loaded Cyclodextrin Nanosponges displayed greater antioxidant activity while trans-Cinnamaldehyde loaded Cyclodextrin Nanosponges exhibited superior antimicrobial activity.Trans-Cinnamaldehyde loadedβCyclodextrin Nanosponges demonstrated significant inhibition(47.66±0.51 mm)against Pseudomonas aeruginosa while Cinnamon Extract loaded β Cyclodextrin Nanosponges exerted maximum inhibition zone against Staphylococcus aureus(27.66±0.51 mm).The current research is important as it compares the efficacy of a pure,isolated bioactive compound with a mixture of bioactive compounds available in the Cinnamon extract.Further,combining bioactive loaded Cyclodextrin Nanosponges with a suitable biopolymer can aid in developing active packaging solutions in the form of film/coating/sachet for preserving highly perishable foods which can significantly benefit the food packaging industry.
