摘要
Lactiplantibacillus plantarum is a nomadic species that displays adaptive variations in different habitats,partic-ularly in the intestines.Bile salts tolerance is crucial for L.plantarum to survive and exert beneficial properties in this environment.L.plantarum exhibits varying growth capacities under bile salts exposure;however,the mo-lecular mechanisms responsible for this variation are not fully understood.In this study,comparative genomics analysis was used to elucidate the specific genes in bile salts-tolerant L.plantarum and further investigate the mechanisms that contribute to bacterial survival under bile salts.A total of eighty-seven specific genes were identified in the tolerant strains,which were primarily associated with the two-component system and carbo-hydrate metabolism.Notably,the mutation of a response regulator(agrA)in the two-component system decreased the growth rate of L.plantarum under bile salts exposure,whereas complementation increased tolerance.Metabolomic analysis suggested that the deletion of agrA resulted in the downregulation of pathways associated with bacterial energy metabolism.These findings indicate that L.plantarum responds to harsh envi-ronments by sensing bile salts as signaling molecules and subsequently adjusting complex metabolic pathways as a survival strategy.This provides novel insights into the molecular basis of the two-component system contributing to bile salts tolerance.
基金
supported by the National Natural Science Foundation of China[No.U23A20259,32394051 and 32122067]
supported by the Fundamental Research Funds for the Central Universities JUSRP622013
Collaborative innovation center of food safety and quality control in Jiangsu Province.
