摘要
Obesity is defined as abnormal or excessive accumulation of body fat,which always causes serious harm to body health.In this work,an anti-obesity strain,Lactiplantibacillus plantarum BHP03(L.plantarum),was explored.This strain showed good reproductive and acid production abilities.And its simulated intestinal fluid tolerance was better than that of Lacticaseibacillus rhamnosus GG(LGG).The in vitro lipid-lowering indicators(inhibition rate of pancreatic lipase,bile-acid salt binding capacity)of L.plantarum BHP03 were at the same level as LGG,but L.plantarum BHP03 exhibited better ability to clear cholesterol.Further,L.plantarum BHP03 was used to intervene high-fat diet mice by oral administration.The results showed that high(10^(9) CFU/day)and low(10^(8) CFU/day)doses of L.plantarum BHP03 could alleviate excessive weight gain caused by high-fat diet and improve the symptoms of hepatorenal enlargement and liver fatty lesions.In addition,high dose L.plantarum BHP03 significantly reduced the content of the serum total cholesterol and low-density lipoprotein cholesterol in mice.Moreover,high dose L.plantarum BHP03 enhanced the species richness of intestinal flora in mice fed with high-fat diet,decreasing the abundance of harmful bacteria such as Staphylococcus and increasing the abundance of beneficial bacteria such as Dubosiella,Akkermansia,Bifidobacterium and Lactobacillus.Therefore,it could be concluded that high dose L.plantarum BHP03 was more effective in preventing obesity and balancing gut microbiota.These findings may provide a theoretical basis for the application of lactobacilli to develop func-tional foods related to anti-obesity.
基金
supported by the Food Engineering Technology Research Center/Key Laboratory of Henan Province[grant number GO202309]
Key Research and Development Program of Henan Province[grant number222102110080]
Henan Province“Double First-Class"Project-Food Science and Technology
National Natural Science Foundation of China[grant number 31701542]
Innovative Funds Plan of Henan University of Technology[grant number2020ZKCJ19]
Cultivation Programme for Young Backbone Teachers in Henan University of Technology.
