摘要
Folate and vitamin D are essential micronutrients,with emerging evidence linking vitamin D and its receptor(VDR)to folate metabolism.This study examined the effect of a folate bio-enriched fermented beverage(BFWB)on vitamin D receptor(VDR)expression and folate transporters activity in colon,using both in vitro and in vivo methods.The BFWB was obtained using two folate-producing strains-Streptococcus thermophilus TH-4 and Bifidobacterium longum subsp.infantis BB-02-cultured in a whey-based matrix supplemented with grape byproduct extract.HCT-116 cell line was treated with the beverage or controls,and mRNA expression levels of vdr,rfc(reduced folate carrier)and pcft(proton-coupled folate transporter)genes were analyzed using quantitative PCR.In the in vivo study,a prevention model was conducted with BALB/c mice fed a folate-depleted diet for 21 days to evaluate whether the BFWB,with or without vitamin D3(2000 IU),could prevent folate deficiency.Folate status and mRNA expression of vdr,rfc,and pcft genes in colonic tissue were also compared to control groups.Results from the animal model revealed that a folate-deficient diet downregulated vdr expression(0.2±0.04-fold change)in the murine colon compared to the control(1.0±0.2).In the HCT-116 cell culture model,the BFWB significantly upregulated the expression of vdr,along with folate transporter pcft(p<0.05).However,in the animal model,vdr expression increased only in the BFWB supplemented with vitamin D3 group(2.9±0.6).Additionally,this study indicated that high ash limited folate absorption from BFWB.
基金
financially supported by the Fundação de Amparo a Pesquisa do Estado de São Paulo(FAPESP#2018/12190-2)
the Food Research Center(FoRC-FAPESP#2013/07914-8)
Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET#PIP 2021-2062 and#PIP 2022-0280)
Agencia Nacional de Promoción de la Investigación,el Desarrollo Tecnológico y la Innovación,(ANPCyT#PICT 2019-0942 and#PICT-2021-CAT-II-00071)
developed within the scope of the CCD-Circula Project(FAPESP#2021/11967-6)
the authors by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(CAPES#88887.473569/2020-00 and 88887.694241/2022-00)
Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq#308696/2022).
