摘要
Adipose thermogenesis activation has emerged as an important target for weight loss at present.Astaxanthin,renowned for antioxidant properties within carotenoid family,is posited to combat obesity.However,its role in thermogenesis remains uncertain.Thus,the thermogenesis efficacy and underlying mechanisms involved in antiobesity of astaxanthin on high-fat diet(HFD)-induced obese mice were explored via biochemical,gut microbiome,and serum metabolomics analysis.The findings indicated that astaxanthin treatment markedly alleviated obesity by highly restoring body weight gain and fat deposition.Notably,there was a remarkable reduction in the mass of brown adipose tissue(BAT)and white adipose tissue(WAT)by 38.90%and 38.52%,respectively(P<0.05).Moreover,astaxanthin induced BAT thermogenesis and WAT beiging as evidenced by critical marker genes,such as Uncoupling Protein 1(UCP1),and PR domain containing 16(PRDM16).Specifically,astaxanthin supplementation significantly increased the expression level of UCP1 by 8.77-fold in BAT and 2.32-fold in WAT.Further microbiota analysis revealed that astaxanthin regulated adipose thermogenesis by enriching specific bacteria,including Alistipes,Alloprevotella,and Prevotella.Additionally,metabolomics analysis identified 3-methyladenine,pyridoxal phosphate,phosphoenolpyruvate,isoleucine,and pyruvate as key metabolites closely related to thermogenesis,participating in carbohydrate,amino acid,and cofactor and vitamin metabolism.Ultimately,Pearson correlation analysis demonstrated astaxanthin modulated serum metabolites through enhancing thermogenesis-related bacteria,highlighting that pyruvate-producer Alistipes may enhance the thermogenic effects mediated by astaxanthin(P<0.05).Overall,this study provides new evidence supporting astaxanthin as a potential driver of thermogenesis,which crosstalk between the intestinal microbiome and metabolome in combating obesity.
基金
financially supported by the National Natural Science Foundation of China(No.32172195)
the Guangzhou Key R&D Plan Project(No.SL2022B03J00806)
the program for Guangdong Introducing Innovative and Entrepreneurial Teams(No.2019ZT08N291).
