摘要
Excessive chloramphenicol(CAP)residue in aquatic products poses major health risks to consumers.Current CAP chromatographic detection technology is accurate,but it has limited on-site rapid detection.As dual-mode sensors are more adaptable for on-site detection,a catalytic system based on a peroxidase-active MIL-101/Au ETHH NR for CAP detection and structural degradation was developed,combining colorimetric and SERS techniques.The CAP was oxidatively degraded by OH⋅in the system,competing to inhibit the oxidation of TMB in the presence of H_(2)O_(2).The structural degradation of CAP resulted from hydrogen extraction and nitroelimination reactions between CAP and OH⋅,and the catalysis was confirmed by the absence of the C-Cl symmetric stretching SRES fingerprint peak at 616 cm^(-1).The detection limits of established SERS and colorimetric processing were 0.01 nM and 1.5 nM,respectively.This colorimetric/SERS dual-mode assay exhibited good linearity in the ranges of 0.1-10μM/0.0001-1μM,indicating promising prospects for on-site applications.
基金
supported by the National Natural Science Foun-dation of China(NO.32302211,32272414)
Natural Science Founda-tion of Fujian Province(NO.2022J01233199)
Xiamen Natural Science Foundation(NO.3502Z20227055)。
