摘要
Isoflavones,a class of polyphenols commonly found in the human diet,are known for their beneficial effects on the gastrointestinal health.However,their precise effect on intestinal motility remains unclear.This study explored the effects of genistein,puerarin,formononetin,and daidzein on intestinal motility in a rat model of ulcerative colitis induced by dextran sulfate sodium(DSS).Our results showed that isoflavone treatment significantly improved colonic morphology,reduced fecal water content,and regulated the intestinal transit time,thereby effectively reducing diarrhea.Mechanistic insights indicated that these isoflavones increased the expression of serotonin reuptake transporter(SERT)and decreased the levels of 5-hydroxytryptamine(5-HT),which led to a reduction in colonic peristaltic stimulation.Furthermore,they increased the expression of c-kit,a marker of interstitial cells of Cajal(ICCs),potentially alleviating motility dysfunction.Molecular docking studies corroborated these findings,demonstrating that isoflavones enhance 5-HT reuptake by facilitating the stable binding of 5-HT to SERT,a mechanism consistent with the observed reduction in 5-HT levels in vivo.These findings suggest that isoflavones have therapeutic potential for treating intestinal motility disorders associated with colitis,and lay the groundwork for further investigation of their biological activities.
基金
supported by the National Natural Science Foundation of China(32060539)
the Research Project of the State Key Laboratory of Food Science and Technology,China(No.SKLF-KF202217)
the Fund for Postgraduate Innovation of Nanchang University,China(YC2020-S055).
