摘要
Pathogenic and spoilage fungi pose great challenges to food safety,leading to increased attention on the development and application of biologically sourced antimicrobial agents.In this study,an antimicrobial protein SDZ3-1 was isolated from Lactiplantibacillus plantarum ALAC-6 and its antimicrobial spectrum was evaluated,demonstrating broad-spectrum antimicrobial activity with particularly strong inhibition of Candida albicans.The mechanism of fungal inhibition was investigated,and the results revealed that,compared to the control group,the minimum inhibitory concentration(MIC)SDZ3-1 treatment led to significant reactive oxygen species(ROS)generation(p<0.001)and a reduction in the reduced glutathione/glutathione disulfide(GSH/GSSG)ratio to 2.56(p<0.001),which further resulted in ROS accumulation and triggered oxidative stress-induced apoptosis in C.albicans.Transcriptome and RT-qPCR assays showed low expression of glr1 and high expression of mca1,the pro-apoptotic factor nma111,and dhh1.Meanwhile,high expression of the Dhh1 gene was observed within p-bodies,suggesting that p-bodies play a role in the apoptotic process and warranting further research.Addi-tionally,transcriptome functional enrichment analysis showed that the C.albicans cells treated with SDZ3-1 were enriched in the autophagy pathway.In summary,the findings of this study indicate that SDZ3-1 effectively inhibits C.albicans through the apoptotic pathway,accompanied by autophagy.Further research should focus on whether the relationship between apoptosis and autophagy in C.albicans cells is cooperative or antagonistic.
基金
supported by the Inner Mongolia Natural Science Foundation Project[grant number 2020ZD13]
the Inner Mongolia Key Technology Project[grant number 2021GG0083].
