摘要
Enterotoxigenic Escherichia coli(ETEC)is the primary causative agent of diarrheal disease and,in some cases,mortality in infants and children.The objective of this study was to ascertain the potential anti-inflammatory and anti-diarrheal properties of egg-derived bioactive tripeptides(IQW and IRW)in the context of ETEC-induced diarrhea.The findings indicated that the IQW and IRW exerted a modulatory effect on ETEC-induced weight loss and intestinal immune dysfunction.The administration of IQW and IRW directly ameliorated ETEC-induced intestinal morphological damage,restored goblet cell counts and intestinal permeability,and enhanced the mechanical and chemical barrier function of the intestinal mucosa.It is noteworthy that IQW and IRW have the capacity to impede the NF-κB/MAPK signaling pathway,a process that may be associated withβ-arrestin2,a pivotal protein within the CaSR response system.Furthermore,IQW and IRW have been demonstrated to play a regulatory role in metabolic pathways and metabolites,including those of tryptophan,arginine,and phenylal-anine,and their respective metabolites.These findings demonstrated the effect of egg-derived bioactive peptides on ETEC diseases and present a framework for the prospective prevention and therapeutics of related diseases.
基金
supported by Natural Science Foundation of Changsha,China(Grant No.kq2402113)
the Youth Foundation of Guangxi Natural Science Foundation(2021JJB130431)
Scientific Research Fund of Hunan Provincial Education Department(22A0154)
Hunan Poultry Industry Technology System(HARS-06).
