摘要
This study aimed to extract an emulsifying agent from the cellulose-based filtration aid of cold-pressed rapeseed oil.This material is currently a low-value stream but contains phospholipids retained during filtration and proteins from residual seed peel and pulp particles.A range of two-step extraction methods were assessed,with oil removal using diethyl ether(DE)or petroleum ether followed by chloroform-methanol(CM)extraction(2:1,3:2 or 5:4 ratios)or the reversed order.Emulsions prepared with the DE/CM3:2 extract displayed the highest emulsifying activity and stability indexes(35.52 m^(2)/g and 2045.18 min)and similar small mean oil droplet sizes(3μm)to those of whey protein concentrate emulsions.A range of extracted compounds contributed to the DE/CM3:2 functionality,including napins,cruciferins and phospholipids.This study demonstrated for the first time the potential to upcycle this co-product by recovering emulsifying agents.Future studies will focus on optimising the extraction process and yield.
