摘要
Peaches are susceptible to Rhizopus stolonifer infection after harvest,resulting in rapid softening and a marked deterioration in quality,as well as shortened shelf life during storage.Herein,the influences of 2,4-brassinoste-roid(EBR)and EBR+Brassinoconazole(BRZ)treatments on disease decay,softening,cell wall polysaccharide degradation in peach fruit were investigated.The results showed that EBR treatment induced disease resistance,as evidenced by an increment of 1.30-2.01 fold higher firmness of peach fruit,by inhibiting the biosynthesis of water-soluble pectin(WSP)with a reduction of 21.46-30.00%due to the inhibition of the expression and activity of cell wall-degrading enzymes(CWDEs),including polygalacturonase(PG),pectate lyase(PL),andβ-galacto-sidase(β-Gal)and pectinesterase(PE),and the improvement of ionically soluble pectin(ISP),covalently soluble pectin(CSP)and lignin.Moreover,the RNA sequencing results demonstrated that EBR upregulated the tran-scription levels of PpBZR1 with 1.87-2.60-fold and PpBZR2 expression with 5.15-12.05-fold,downregulated the PpGSK3 expression with the reduction of 54.02%and 27.45%in 9h and 12h after treatment.On the contrary,peach fruit following EBR+BRZ treatment exhibited opposite effects in the PpBZR1/2 and PpGSK3 expression of RNA-seq and cell wall polysaccharides degradation.Based on these results,EBR treatment reduced disease decay and delayed softening by influencing cell wall polysaccharide disassembly,which might be regulated by PpGSK3 phosphorylation and PpBZR1/2 transcription regulation in postharvest peaches.Therefore,EBR treatment would be a potential method in extending postharvest shelf life of other climacteric fruits.
基金
funded by the Youth Program of National Natural Science Foundation of China(32402189)
HN HautFood IAEG-038,the Research Foundation for High-level Talents of Henan University of Technology(2024BS028)
the Natural Science Foundation of Henan Province(242300421200)
The Fund of Food Engineering Technology Research Center/Key Laboratory of Henan Province,Henan University of Technology(Grant No.GO202307 and Grant No.GO202308)
“Double First-class”Undergraduate Science and Technology Innovation Ability Promotion Project of Henan University of Technology(HNHautFood IAEM-011).
