摘要
柑橘是世界第一大水果,通常认为其细胞质遵循母系遗传规律.但最近研究发现,柑橘杂交后代的细胞质并非完全遗传自母系亲本,目前柑橘杂交群体的细胞质遗传规律尚不清楚.本研究从自然群体材料细胞器基因组系统发育关系分析中发现了柑橘细胞器基因组的多样性、核质进化冲突以及柑橘杂交过程中细胞器基因组存在非典型现象.为进一步探究柑橘杂交群体的细胞质遗传规律,本研究以宽皮柑橘“CR038”(Citrus reticulata)为母本,枳“Pt005”(C.trifoliata)为父本创制了柑橘F 1杂交群体.利用HiFi数据组装了群体亲本的完整细胞器基因组(mitogenome和chlorogenome),并利用重测序数据组装了杂交后代的细胞器基因组草图.通过分析和鉴定杂交群体细胞器基因组的群体结构和重组事件,将柑橘杂交群体的细胞质遗传方式划分为母系遗传、双亲遗传和类父系遗传3种类型.本研究基于全基因组分析首次揭示了柑橘杂交群体的细胞质遗传规律,为柑橘细胞质研究提供了新的视角,对柑橘遗传育种具有重要意义.
Citrus is one of the most essential fruit crops worldwide,and it is generally considered that its cytoplasmic information is inherited from the maternal parent.Recent research has indicated that not all cytoplasmic genomes of citrus hybrids are derived from their female parent.However,the cytoplasmic inheritance pattern in citrus hybridization remains largely unexplored.In this study,we characterized the organelle genome diversity,nuclear-cytoplasmic evolutionary divergence and atypical genetic events in organelle genomes during hybridization in citrus by analyzing the phylogenetic relationship of organelle genomes in a natural citrus population.An F 1 population was created from the cross with“CR038”(Citrus reticulata)as the maternal parent and“Pt005”(C.trifoliata)as the paternal parent.The complete organellar genomes(mitochondria and chloroplasts)of the parents were assembled using HiFi reads,and the draft organellar genomes of the offspring were also assembled using resequencing data.According to the analysis and identification results on phylogenetic relationship,comparative genome and recombination events,the cytoplasmic inheritance pattern of citrus could be classified into three types:maternal inheritance,biparental inheritance,and pseudo-paternal inheritance.This study is the first to reveal the cytoplasmic inheritance pattern in citrus based on the whole genome sequence data,which provides a novel insight into the genetic breeding in citrus.
作者
孙晓峰
王小涵
岳红丽
李明祝
王福生
江东
朱世平
SUN XiaoFeng;WANG XiaoHan;YUE HongLi;LI MingZhu;WANG FuSheng;JIANG Dong;ZHU ShiPing(Citrus Research Institute,Southwest University,Chongqing 400712,China;Citrus Research Institute,Chinese Academy of Agricultural Sciences,Chongqing 400712,China;National Citrus Engineering Research Center,Chongqing 400712,China)
出处
《中国科学:生命科学》
北大核心
2026年第3期657-670,共14页
Scientia Sinica(Vitae)
基金
国家重点研发计划(批准号:2023YFD1200103)
国家柑橘产业体系(批准号:CARS-Citrus)
中央高校基本科研业务费专项资金(批准号:SWU-XDJH202308)资助。
关键词
柑橘
细胞器基因组
杂交群体
核质进化冲突
细胞质遗传
citrus
organellar genome
hybrid population
nuclear-cytoplasmic conflict
cytoplasmic inheritance
