摘要
Postoperative abdominal adhesion,coupled with adverse effects,threatens to patient's life.However,most bioadhesives as anti-tissue adhesion barrier encounter unreliable adhesion towards slippery abdominal wall along with accidental mispositioning during use,even overlook the impact of frictional stimuli and inflammation on abdominal adhesion.Herein,inspired by lubricated peritoneum,a programmable adhesive dual-layer Janus patch(DJP)barrier with unilateral lubrication and anti-inflammation integrating lubricated layer and adhesive matrix is developed to prevent postoperative abdominal adhesion.Programmable adhesion of DJP rapidly establishes adhesion interface between barrier and tissue primarily through noncovalent interaction,then enhances the interfacial stability of 2.81-fold through covalent interaction.This timescale-dependent adhesion can also allow the mispositioned bioadhesive to be repositioned on tissue in short time,improving surgical fault tolerance.Hydration of micron-scale poly sulfobetaine methacrylamide brush on DJP surface imitates peritoneal lubrication with low coefficient of friction(0.06),diminishing frictional stimuli towards injured tissue.Meanwhile,anti-inflammation of DJP by the antioxidative catechol-containing copolymer is demonstrated in vitro.Further,a rat model indicates that DJP adhering to injured site reduces deposited collagen between abdominal wall and cecum,preventing abdominal adhesion and facilitating tissue healing compared with commercial barriers.Overall,this work provides a notable guiding reference in development of antiadhesive biomaterials.
术后腹部粘连伴随不良反应,威胁患者健康.多数防粘连黏附屏障对湿滑腹壁黏附不佳、易定位错误,且忽略摩擦刺激、炎症对粘连的影响.受腹膜启迪,本研究将润滑与黏附结合,设计兼具程序黏附、抗炎的单侧润滑Janus屏障(DJP),预防腹部粘连. DJP的程序黏附可由非共价作用与组织迅速建立黏附,再由共价键将稳定性提高2.81倍.程序黏附使材料在短时间内能再定位,提高容错率. DJP表面聚磺酸甜菜碱刷模拟腹膜水合润滑,具有低摩擦系数(0.06),可缓解对组织的摩擦刺激;体外结果表明DJP含抗氧化儿茶酚基聚合物,可减轻炎症.最终,体内实验证明,相比商用屏障, DJP可减少腹壁、盲肠间胶原沉积,预防腹部粘连,促进愈合.本工作可为防粘连生物材料研究提供参考.
基金
supported by the National Natural Science Foundation of China (U22A20158 and 52273135)
Sichuan Science and Technology Program (2025ZNSFSC0338)
China Postdoctoral Science Foundation (GZC20241140)
State Key Laboratory of Advanced Polymer Materials (sklpme 2023-2-16)。
