摘要
目的 基于网络药理学和动物实验,探讨麦粒灸(Moxibustion with seed-sized moxa cones,MSMC)中黄酮类化合物治疗周围神经损伤(Peripheral nerve injury,PNI)的潜在作用机制。方法 通过TCMSP数据库筛选MSMC的活性成分,从GeneCards、OMIM、TTD数据库获取PNI相关靶点,取交集后构建蛋白互作(Protein-protein interaction network,PPI)网络,筛选核心靶点并进行GO和KEGG富集分析。采用分子对接验证活性成分与靶点的结合特性,并建立大鼠坐骨神经损伤模型进行实验验证。结果 从MSMC中筛选出135个黄酮类活性成分,对应1576个靶点。GO分析显示,共同靶点主要富集于氧化应激反应和炎症调控等生物过程。KEGG分析提示,PI3K-AKT等5条关键信号通路可能参与作用机制。分子对接显示,黄酮类化合物与核心靶点具有较强的结合能力和稳定的复合物结构。动物实验显示,与模型组相比,MSMC治疗可改善坐骨神经功能指数(Sciatic functional index,SFI)(P<0.05),提升神经元结构完整性和数量,降低Fe2+和丙二醛(Malondialdehyde,MDA)水平,提高谷胱甘肽(Glutathione,GSH)水平(P<0.05)。结论 MSMC中的黄酮类化合物可能通过抗氧化应激作用改善PNI症状,促进神经修复再生。
Objective To investigate the potential mechanism of flavonoids in moxibustion with seed-sized moxa cones(MSMC)for peripheral nerve injury(PNI)Methods Active components of MSMC were screened using the T rbaadsietido noanl Cnheitnweosrek M pehdaicrminaec Soylosgteym asn Pdh aarnmimacaoll oegxy(p eriments.TCMSP)database,while PNI-related targets were retrieved from GeneCards,Online Mendelian Inheritance in Man(OMIM),and Therapeutic Target Database(TTD).After the intersection,a protein-protein interaction(PPI)network was constructed,followed by core target screening and enrichment analyses,including Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).Then,molecular docking was performed to validate the binding properties between active components and the targets.Finally,an experimental rat model of sciatic nerve injury was built for validation.Results A total of 135 flavonoid active components corresponding to 1,576 targets were screened from MSMC.GO analysis indicated that shared targets were primarily enriched in biological processes such as oxidative stress response and inflammatory regulation.KEGG analysis suggested that the mechanism might involve five key signaling pathways,including phosphoinositol 3 kinase-protein kinase B(PI3K-AKT).Molecular docking demonstrated strong binding affinity and stable complex structure between flavonoid compounds and core targets.Animal experiments revealed that compared to the model group,MSMC treatment significantly improved the sciatic functional index(SFI)(P<0.05),neuronal structural integrity,and quantity,reduced Fe2+and malondialdehyde(MDA)levels,and increased glutathione(GSH)levels(P<0.05).Conclusion Flavonoid compounds in MSMC can alleviate PNI symptoms and promote neural repair and regeneration via antioxidative stress.
作者
苗龙杰
秦晓彬
李征
陈双丽
邓程硕
周望高
余少校
叶学浪
庄加川
MIAO Long-jie;QIN Xiao-bin;LI Zheng;CHEN Shuang-li;DENG Cheng-shuo;ZHOU Wang-gao;YU Shao-xiao;YE Xue-lang;ZHUANG Jia-chuan(Shenzhen Clinical College of Integrated Chinese and Western Medicine,Guangzhou University of Chinese Medicine,Shenzhen Guangdong 518104;Shenzhen Integrated Traditional Chinese and Western Medicine Hospital,Shenzhen Guangdong 518104)
出处
《世界中西医结合杂志》
2025年第11期2193-2203,共11页
World Journal of Integrated Traditional and Western Medicine
基金
宝安区中医药临床研究专项立项项目(2023ZYYLCZX-3)
深圳市宝安区属公立医院高质量发展研究项目(BAGZL2024048)。
关键词
周围神经损伤
黄酮类化合物
网络药理学
麦粒灸
中医传统治疗
Peripheral Nerve Injury
Flavonoid Compound
Network Pharmacology
Moxibustion with Seed-Sized Moxa Cone
Traditional Chinese Medicine Treatment
