摘要
目的分析不同类型样本人巨细胞病毒(HCMV)核酸检测在HCMV感染中的价值。方法收集2022—2023年青海红十字医院疑似HCMV感染者乳汁、羊水,以及乳汁HCMV-DNA阳性对应新生儿晨尿、痰液、血清、脑脊液等样本,采用荧光定量聚合酶链反应(PCR)进行检测,比较不同类型样本HCMV阳性率差异。结果所有类型样本中,乳汁HCMV检出率最高(67.93%),乳汁HCMV-DNA阳性对应新生儿尿液、痰液、血清、脑脊液HCMV阳性率分别为16.92%、12.50%、2.64%、0.12%,差异有统计学意义(P<0.001);不同性别不同类型样本HCMV阳性率差异无统计学意义(P>0.05)。有115例≤30 d新生儿血清(21例)、脑脊液(1例)、痰液(20例)、尿液(73例)样本HCMV-DNA阳性。结论采用荧光定量PCR技术检测羊水样本HCMV-DNA,有助于尽早判断宫内感染,预防新生儿HCMV感染。
Objective To analyze the roles of different specimen types for human cytomegalovirus(HCMV)nucleic acid testing.Methods Specimens such as breast milk,amniotic fluid,morning urine,sputum,serum and cerebrospinal fluid from suspected HCMV-infected or breast milk HCMV-DNA positive infants at Qinghai Red Cross Hospital from 2022 to 2023 were collected.Fluorescence quantitative polymerase chain reaction(PCR)was used,and the differences in HCMV positive rates among different specimen types were compared.Results Among all the specimen types,the HCMV determination rate in breast milk was the highest(67.93%).The HCMV positive rates of urine,sputum,serum and cerebrospinal fluid of the corresponding breast milk HCMV-DNA positive infants were 16.92%,12.50%,2.64%and 0.12%,respectively(P<0.001).There was no statistical significance in HCMV positive rates among infants with different genders and for different specimen types(P>0.05).There were 115 cases of HCMV-DNA positive in the serum(21 cases),cerebrospinal fluid(1 case),sputum(20 cases)and urine(73 cases)of≤30 d infants.Conclusions Using qPCR to determine HCMV-DNA amniotic fluid can provide a reference for early diagnosis of intrauterine infection to prevent neonatal infection.
作者
王春玮
马秀清
齐国昌
蔡星垚
WANG Chunwei;MA Xiuqing;QI Guochang;CAI Xingyao(Department of Clinical Laboratory,Qinghai Red Cross Hospital,Xining 810000,Qinghai,China)
出处
《检验医学》
2025年第9期881-884,共4页
Laboratory Medicine
基金
青海省卫生健康委员会指导性计划课题(2022-wjzdx-93)。
关键词
人巨细胞病毒
乳汁
羊水
核酸检测
新生儿
Human cytomegalovirus
Breast milk
Amniotic fluid
Nucleic acid testing
Infant
