摘要
为研究聚苯乙烯微塑料对褐菖鲉(Sebastiscus marmoratus)肠道的毒性效应,将褐菖鲉分别暴露于0、1和10 mg/L的聚苯乙烯微塑料溶液中21 d,通过富集试验、组织学切片、转录组学及16S rRNA测序等技术检测褐菖鲉肠道形态结构、差异表达基因以及微生物菌群的变化。结果表明,褐菖鲉肠道中聚苯乙烯微塑料积累量随着暴露时间的增加呈现显著性上升趋势,7 d时质量分数达到16.20×10^(−6)(μg/g)。聚苯乙烯微塑料暴露会对肠道造成病理学损伤,1 mg/L质量浓度组表现为肠黏膜细胞出现坏死和脱落并伴有空泡结构形成,10 mg/L质量浓度组肠绒毛出现更为明显萎缩和坏死,空泡化结构增多,肠壁厚度、肌层厚度、肠绒毛长度和宽度均出现显著性降低。转录组测序显示聚苯乙烯微塑料暴露后7 d和21 d各有313和169个基因的表达量出现显著性变化,经KEGG富集分析发现7 d时主要富集在p53信号通路(p53 signaling pathway)、淀粉和蔗糖代谢途径(starch and sucrose metabolism)、Toll样受体信号通路(Toll-like receptor signaling pathway)等;21 d时主要富集在类固醇生物合成途径(Steroid biosynthesis)、花生四烯酸代谢途径(arachidonic acid metabolism)、NOD样受体信号通路(NOD-like receptor signaling pathway)等。肠道微生物菌群结构在门分类水平上不会随着聚苯乙烯微塑料暴露发生明显变化,但是梭杆菌科(Fusobacteriaceae)、鲸杆菌属(Cetobacterium)、弧菌科(Vibrionaceae)和普雷沃氏菌属(Prevotella)等相对丰度增加也暗示着肠道屏障修复和抗炎症功能升高。综上所述,聚苯乙烯微塑料暴露可损伤褐菖鲉肠道组织,破坏肠道屏障并诱发炎症,进而影响其健康。
To evaluate the toxicological effects of polystyrene microplastics(PS-MPs)on the intestinal health of Sebastiscus marmoratus,individuals were exposed to PS-MP solutions at concentrations of 0,1,and 10 mg/L for 21 days.A combination of analytical approaches,including enrichment analysis,histological examination,transcriptomic profiling,and 16S rRNA gene sequencing,was employed to assess alterations in intestinal morphology,gene expression,and microbial community composition.The results demonstrated a time-dependent accumulation of PSMPs in the intestines,with mass fraction reaching 16.20×10^(–6)(μg/g)by day 7.Histopathological analysis revealed dose-dependent intestinal damage:at 1 mg/L,necrosis,detachment,and vacuolar degeneration of mucosal cells were observed;at 10 mg/L,severe villus atrophy,necrosis,vacuolization,and significant reductions in intestinal wall thickness,muscle layer thickness,and villus length and width were evident.Transcriptomic analysis identified 313 and 169 differentially expressed genes(DEGs)after 7 and 21 days of exposure,respectively.KEGG pathway enrichment revealed that DEGs at day 7 were primarily involved in the p53 signaling pathway,starch and sucrose metabolism,and Toll-like receptor signaling pathway.By day 21,enrichment was observed in pathways related to steroid biosynthesis,arachidonic acid metabolism,and NOD-like receptor signaling pathway.Although no signific-ant changes in microbial composition were detected at the phylum level,notable increases in the relative abundances of Fusobacteriaceae,Vibrionaceae at the family level and Cetobacterium,Prevotella at the genus level were observed,potentially indicating enhanced intestinal barrier repair and anti-inflammatory responses.In conclusion,PS-MP exposure resulted in structural damage to intestinal tissues,disruption of the mucosal barrier,and inflammatory responses in S.marmoratus,ultimately compromising organismal health.
作者
罗统钦
阮泽超
张燕
王跃斌
汪倩凤
柴学军
Luo Tongqin;Ruan Zechao;Zhang Yan;Wang Yuebin;Wang Qianfeng;Chai Xuejun(Fisheries College,Zhejiang Ocean University,Zhoushan 316022,China;Key Laboratory of Mariculturre&Enhancement of Zhejiang Province,Zhoushan 316021,China)
出处
《海洋学报》
北大核心
2025年第9期115-128,共14页
基金
浙江省海洋水产研究所科技计划项目院所专项(HYS-CZ-202504)
浙江省海洋水产研究所渔业专项(HYS-CZ-202506)
舟山市育种育苗科创中心专项(2024Y001-4)。
