摘要
【目的】为了解鄱阳湖周边地区流浪犬犬细小病毒(canine parvovirus,CPV)流行情况与CPV遗传变异特征。【方法】于2023年1—6月从鄱阳湖周边地区共采集200份流浪犬粪便样品,使用胶体金试纸进行抗原检测初筛,选取10份阳性样品进行PCR分段扩增CPV全长基因,并测定核苷酸序列,利用生物信息软件分析VP2基因的氨基酸序列并构建进化树,进行同源性差异分析以及氨基酸变异位点分析。【结果】胶体金抗原检测共发现56份阳性粪便,阳性率为28%,选取10份阳性粪便进行基因分段扩增,共获得10株CPV全长序列,分别命名为CPV-NC-2、CPV-NC-7、CPV-NC-8、CPV-NC-9、CPV-NC-17、CPV-NC-29、CPV-NC-31、CPV-NC-32、CPV-NC-34、CPV-NC-35,拼接后对CPV VP2基因进行序列分析,与25株CPV参考毒株VP2序列对比发现,氨基酸序列同源性在98.4%~99.7%,各毒株之间存在多个氨基酸位点变异,进化树分析发现共有8株属于2c亚型,2株属于2a亚型。【结论】鄱阳湖周边地区发现流行的犬细小病毒(CPV)包含两种亚型,并检测到病毒变异现象,相关结果为该地区犬细小病毒的防控工作提供了科学依据。
[Objective]This study aimed to understand the prevalence of canine parvovirus(CPV)and the genetic variation characteristics of CPV in stray dogs around the Poyang Lake.[Method]This study was conducted in January to June 2023 to collect a total number of 200 fecal samples from stray dogs in the Poyang Lake area.Colloidal gold immunochromatographic tests were used for the preliminary screening of antigens,from which 10 positive samples were selected for PCR amplification of the full-length CPV gene.The nucleotide sequences were determined,and bioinformatics software was used to analyze the amino acid sequences of the VP2 gene and construct a phylogenetic tree,followed by homology difference analysis and amino acid variation site analysis.[Result]Results showed that 56 positive fecal samples were identified through the colloidal gold antigen test,resulting in a positivity rate of 28%.Gene segment amplification yielded 10 full-length CPV sequences,named CPV-NC-2,CPV-NC-7,CPV-NC-8,CPV-NC-9,CPV-NC-17,CPV-NC-29,CPV-NC-31,CPV-NC-32,CPV-NC-34,and CPV-NC-35.Sequence analysis of the VP2 gene indicated that the amino acid sequence homology ranged from 98.4% to 99.7%.Multiple amino acid residue variations were observed among the strains.Phylogenetic analysis revealed that 8 strains belonged to the 2c subtype,while 2 strains belonged to the 2a subtype.[Conclusion]This study indicates that two subtypes of CPV are prevalent in the Poyang Lake area,and mutations have occurred.This study provides data support for the prevention and control of canine parvovirus in the vicinity of the Poyang Lake.
作者
刘琪
黄漫孜
查博骞
吴虹瑾
肖艳
邓海欣
曾庆华
梁惠平
吴欢生
LIU Qi;HUANG Manzi;CHA Boqian;WU Hongjin;XIAO Yan;DENG Haixin;ZENG Qinghua;LIANG Huiping;WU Huansheng(Key Laboratory of Livestock and Poultry Disease Diagnosis of Jiangxi Province/Institute of Livestock and Poultry Disease Control,School of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《生物灾害科学》
2025年第3期363-370,共8页
Biological Disaster Science
基金
江西省现代农业产业体系项目(JXARS-09)。
关键词
犬细小病毒
流行病学调查
VP2基因
遗传进化
序列分析
canine parvovirus
epidemiological investigation
VP2 gene
genetic evolution
sequence analysis
