摘要
目的以气候法叠加流感病毒滴鼻,筛选建立甲型H1N1流感风热证小鼠模型。方法将72只BALB/c小鼠随机分为9组:对照(Control)组,风热(FR)组(FR-3Day组、FR-5Day组),模型(Model)组(1LD-3Day组、2LD-3Day组、3LD-3Day组、1LD-5Day组、2LD-5Day组、3LD-5Day组),每组8只。Control组正常环境饲养,FR组和Model组风热条件(温度37℃,湿度50%,有微风,正常室内光照,8 h/d)干预7 d后,第8天进行PR8流感病毒滴鼻感染,Control组、FR组小鼠使用等量生理盐水滴鼻。攻毒后各组均在正常环境下饲养,并分别在第3、5天取材。观察记录小鼠外观及行为状态以及大便、体温,检测各组小鼠肺指数、血常规、肺部组织病理、血清IL-6水平及病毒滴度。结果与Control组相比,FR组小鼠风热干预7 d后,外观、大便、体温、血常规及肺组织病理均无明显异常;Model组与各组相比,随着攻毒量的增加及时间的延长,小鼠外观、肺指数、RBC、HGB、HCT、病理结果、体温各指标进行性加重;同一攻毒量时,NEUTP、LYMPHP、病毒滴度、血清中IL-6严重程度在攻毒后第3天达到顶峰,攻毒后第5天略有减轻。结论风热气候干预7 d后予PR8滴鼻攻毒制备流感风热证小鼠模型具有可行性。
Objective To establish a mouse model of H1N1 influenza wind-heat syndrome by combining climate intervention with influenza virus nasal drops.MethodsSeventy-two BALB/c mice were divided randomly into nine groups:a Control group,wind-heat(FR)groups(FR-3Day,FR-5Day),and Model groups(1LD-3Day,2LD-3Day,3LD-3Day,1LD-5Day,2LD-5Day,2LD-5Day,3LD-5Day)(n=8 mice per group).Mice in the Control group were housed in a normal environment,while mice in the FR and Model groups were kept in wind-heat conditions for 7 d.Mice in the Model groups received nasal PR8 influenza virus infection on the 8th day,and mice in the Control and FR heat groups received equal amounts of physiological saline nasal drops.After virus challenge,each group was housed in a normal environment and samples were taken on days 3 and 5.The appearance of the mice was observed and recorded and the lung index,routine blood parameters,lung tissue pathology,serum interleukin(IL)-6 levels,and virus titers were detected in each group based on their behavioral status,stools,and body temperature.ResultsAfter 7 d of wind-heat intervention,mice in the FR groups showed no significant abnormalities in terms of appearance,stools,body temperature,routine blood parameters,or lung tissue pathology compared with the Control group.The appearance,lung index,red blood cell count,hemoglobin,hematocrit,pathological result,and body temperature in the Model groups worsened progressively with increasing time and toxin dosage,while the neutrophil percentage,lymphocyte percentage,virus titer,and serum IL-6 levels peaked on day 3 after viral attack,for the same viral dose,and then decreased slightly on day 5.ConclusionsPR8 nasal drops and 7 d of wind-heat climate intervention can be used to establish a mouse model of influenza wind-heat syndrome.
作者
张晓艳
谢苗
胡齐帅
冯鑫鑫
王玉涛
赵昕
梁燕莉
陈林洋
杨子峰
ZHANG Xiaoyan;XIE Miao;HU Qishuai;FENG Xinxin;WANG Yutao;ZHAO Xin;LIANG Yanli;CHEN Linyang;YANG Zifeng(Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;Guangzhou Medical University,Guangzhou 511436,China;Institute of Analysis,Guangdong Academy of Sciences(Guangzhou Analytical Testing Center,China),Guangzhou 510070,China;the First Affiliated Hospital of Guangzhou Medical University,Guangzhou Institute of Respiratory Health,State Key Laboratory of Respiratory Disease,National Clinical Research Center for Respirator Disease,Guangzhou 510120,China)
出处
《中国实验动物学报》
北大核心
2025年第8期1105-1115,共11页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金青年基金项目(81803971)
2019年度河南省高等学校青年骨干教师培养计划项目(2019GGJS111)
2018年度河南省高等学校重点科研项目指导计划项目(18B360009)。
关键词
流感
风热证
动物模型
病证结合
influenza
wind-heat syndrome
animal models
combination of disease and syndrome
