摘要
采用超高效液相色谱-四极杆-飞行时间质谱(UPLC-QTOF-MS)联用技术分析复方灯台叶片的化学成分。根据化合物的质谱数据,并结合对照品及文献报道,共鉴定或指认出52个成分,包括12个生物碱类化合物、13个萜类化合物、14个黄酮类化合物、3个酚酸类成分,以及10个其他化学成分(如二苯乙烯类、有机酸类)。通过建立小鼠气管酚红分泌模型、氨水引咳模型、豚鼠磷酸组胺联合氯化乙酰胆碱引喘模型,评价了复方灯台叶片的祛痰、止咳、平喘药效。结果显示,与模型组相比,复方灯台叶片的中、高剂量(2、4 g/kg)治疗组小鼠气管酚红分泌量显著增加,咳嗽潜伏期显著延长,2 min内咳嗽次数显著下降(P<0.05,P<0.01);豚鼠引喘潜伏期延长。该研究阐明了复方灯台叶片的化学成分组成,评价了祛痰、止咳、平喘作用,为其质量控制和临床应用提供了试验依据。
A UPLC-QTOF-MS method was established to analyze the chemical constituents of Fufang Dengtaiye tablets.Based on the mass spectrometry data,reference substances,and literature reports,a total of 52 constituents were identified or speculated.The chemical material basis was composed of 12 alkaloids,13 terpenoids,14 flavonoids,3 phenolic acids,and 10 other chemical constituents(e.g.,stilbenes and organic acids).The phenol red-induced tracheal excretion mouse models,ammonia-induced mouse cough models,and histamine phosphate combined with acetylcholine chloride-induced guinea pigs asthma models were established to evaluate the expectorant,antitussive and anti-asthmatic effects of Fufang Dengtaiye tablets.The results showed that compared with the model group,the medium-and high-dosage(2 and 4 g/kg)group of Fufang Dengtaiye tablets could significantly increase the secretion of phenol red,prolong the cough incubation period,reduce the number of cough in two minutes of mice(P<0.05,P<0.01),and increase asthma incubation period of guinea pigs.This study elucidated the chemical constituents of Fufang Dengtaiye tablets and its expectorant,antitussive and antiasthmatic activities,which provided a experimental basis for its quality control and clinical application.
作者
周锦
聂岚岚
崔明筠
付开聪
胡晓
黄春跃
侯爱君
ZHOU Jin;NIE Lanlan;CUI Mingjun;FU Kaicong;HU Xiao;HUANG Chunyue;HOU Aijun(National Key Lab.of Lead Druggability Research,Shanghai Institute of Pharmaceutical Industry Co.,Ltd.,China State Institute of Pharmaceutical Industry,Shanghai 201203;School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240;Puer National Institute of Ethnic Traditional Medicine,Puer 665000)
出处
《中国医药工业杂志》
2025年第5期643-652,共10页
Chinese Journal of Pharmaceuticals
基金
上海市“科技创新行动计划”生物医药科技支撑专项项目(22S21901800)——治疗慢性支气管炎医疗机构制剂复方灯台叶片的新药临床前研究。
关键词
复方灯台叶片
UPLC-QTOF-MS
化学成分
鉴定
药效学
祛痰作用
止咳作用
平喘作用
Fufang Dengtaiye tablet
UPLC-QTOF-MS
chemical constituent
identification
pharmacodynamics
expectorant effect
antitussive effect
antiasthmatic effect
