摘要
核酮糖-1,5-二磷酸羧化酶/加氧酶(ribulose-1,5-bisphosphate carboxylase/oxygenase,Rubisco)是植物光呼吸过程中的关键酶,在光合作用中决定了碳同化速率。本研究利用NCBI-BLAST、ProtParam、ProtScale、TMHMM、Targetp、SOPMA、SWISS-MODEL、SignalP-5.0、PSORTⅡ、Promoter-2.0、STRING等在线软件,针对牡丹中核酮糖1,5-二磷酸羧化酶(PsRbc)蛋白进行生物信息学分析。结果表明,Ps Rbc蛋白由425个氨基酸组成,pI为6.60,分子量为47088.56 Da,为稳定性较高的亲水性蛋白质;无信号肽区域,无跨膜结构,在细胞质中分布较多;二级结构分析表明Ps Rbc以α-螺旋和无规卷曲为主;系统发育分析表明牡丹与艾菲拉草、美国省沽油、锐尖山香圆、台湾林檎的同源性最高,亲缘关系较近。对Ps Rbc编码蛋白进行生物信息学分析,获得了基因的特征及预测蛋白发挥的功能,为进一步开展牡丹功能基因研究提供了科学依据。
Ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)is a key enzyme in plant photorespiration,which determines the rate of carbon assimilation in photosynthesis.This study used online software such as NCBI-BLAST,ProtParam,ProtScale,TMHMM,Targetp,SOPMA,SWISS-MODEL,SignalP-5.0,PSORT II,Promoter-2.0,STRING,etc.to conduct bioinformatics analysis of ribose 1,5-diphosphate carboxylase(PsRbc)protein in Paeonia suffruticosa.The results showed that PsRbc protein was composed of 425 amino acids with pI of 6.60 and molecular weight of 47088.56 Da.It was a stable hydrophilic protein;there is no signal peptide region,no transmembrane structure,and more distribution in the cytoplasm;the secondary structure analysis shows that PsRbcα-helix and random coil are the main;phylogenetic analysis shows that Paeonia suffruticosa has the highest homology with Leptomeria aphylla,Turpinia arguta,Docynia doumeri,and Staphylea trifolia,and the genetic relationship is relatively close.Through bioinformatics analysis of PsRbc encoded protein,the characteristics of the gene and the function of the predicted protein were obtained,which provided scientific evidence for further research on the functional gene of Paeonia suffruticosa.
作者
许燕
刘自平
黄茸茸
刁欢
王章婕
邸亮
Xu Yan;Liu Ziping;Huang Rongrong;Diao Huan;Wang Zhangjie;Di Liang(College of Pharmacy,Anhui Xinhua University,Hefei,230000;Pharmacy Department of the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine,Hefei,230000)
出处
《分子植物育种》
北大核心
2025年第8期2490-2496,共7页
Molecular Plant Breeding
基金
安徽省教育厅自然科学项目(KJ2020A0790,2022AH051872,2022AH051858)
安徽省教育厅质量工程项目(2020jyxm0785,2021sx066,2021jyxm0613,2020wyxm099)
安徽省大创项目(AH202012216106,AH202112216206)
安徽新华学院校级科研项目(2022zr004)共同资助。
