摘要
目的探究磷脂酶Cβ1(PLCB1)表达是否导致胃腺癌转移和预后恶化,并初步分析其作用机制。方法选取122例胃腺癌患者及其癌旁组织,采用组织微阵列免疫组织化学技术(IHC)检测PLCB1、上皮钙黏蛋白(E-cadherin)、波形蛋白(vimentin)、CD8的表达。根据PLCB1的IHC评分将患者分为PLCB1高表达组(IHC>90)和PLCB1低表达组(IHC≤90)。比较两组患者的临床病理特征、上皮间质转化(EMT)相关蛋白E-cadherin、vimentin和CD8^(+)T细胞的表达差异。收集患者的总生存期,运用COX回归分析和Kaplan-Meier曲线评估PLCB1表达水平与预后的关系。结果胃腺癌组织中PLCB1高表达者占55例,癌旁组织中仅占12例。PLCB1高表达组的肿瘤浸润深度、淋巴结转移程度和TNM分期均高于PLCB1低表达组,PLCB1表达水平与E-cadherin呈负相关(r=-0.339),与vimentin呈正相关(r=0.211),与CD8^(+)T细胞呈负相关(r=-0.343)。Kaplan-Meier曲线分析表明PLCB1高表达的胃癌患者总生存期显著降低。多因素COX回归分析表明除淋巴结转移外,肿瘤浸润深度、TNM分期、E-cadherin和vimentin也是胃癌患者的独立预后因素。结论PLCB1在胃癌中高表达,与肿瘤侵袭和预后差密切相关。PLCB1可能通过诱导EMT和抑制CD8^(+)T细胞浸润影响胃癌转移和免疫反应。
Objective To investigate whether PLCB1 expression leads to gastric adenocarcinoma metastasis and poor prognosis,and to preliminarily analyze its mechanism.Methods 122 gastric adenocarcinoma patients and their adjacent non-cancerous tissues were selected,and tissue microarray technology was used to detect the expression levels of PLCB1,epithelial cadherin(E-cadherin),vimentin and CD8^(+)T cells by immunohistochemistry,and scored by two pathologists.According to the immunohistochemical score of PLCB1,the patients were divided into PLCB1 high expression group(IHC>90)and PLCB1 low expression group(IHC≤90).The clinical pathological characteristics,epithelial mesenchymal transition(EMT)-related proteins and CD8^(+)T cells expression differences between the two groups were compared.The overall survival of the patients was collected,and COX regression analysis and Kaplan-Meier curve were used to evaluate the relationship between PLCB1 expression level and prognosis.Results PLCB1 was highly expressed in 55 cases of gastric adenocarcinoma tissues,while only 12 cases in adjacent non-cancerous tissues.The tumor invasion depth,lymph node metastasis degree and TNM stage of the PLCB1 high expression group were higher than those of the PLCB1 low expression group.Chi-square test showed that PLCB1 expression level was negatively correlated with E-cadherin(r=-0.339),positively correlated with vimentin(r=0.211),and negatively correlated with CD8^(+)T cells(r=-0.343).Kaplan-Meier curve analysis showed that the overall survival and disease-free survival of gastric adenocarcinoma patients with high PLCB1 expression were significantly reduced.Multivariate COX regression analysis showed that except for lymph node metastasis,tumor invasion depth,TNM stage,E-cadherin and vimentin were also independent prognostic factors for gastric adenocarcinoma patients.Conclusion PLCB1 is highly expressed in gastric adenocarcinoma,and is closely related to tumor aggressiveness and prognosis.PLCB1 may induce EMT and inhibit CD8^(+)T cell infiltration to affect gastric adenocarcinoma metastasis and immune response.
作者
岳灵萍
陈俊峰
郜倩倩
YUE Lingping;CHEN Junfeng;GAO Qianqian(Department of Pathology,Changzhou Tumor Hospital Affiliated to Soochow University,Changzhou 213000;Department of Gastrointestinal Surgery,Xijing Hospital of Digestive Diseases,Air Force Medical University,Xi’an 710032,China)
出处
《细胞与分子免疫学杂志》
北大核心
2025年第5期444-449,共6页
Chinese Journal of Cellular and Molecular Immunology
基金
2020年常州市应用基础研究(CJ20200063)
陕西省重点研发计划(2018SF-497)。
