摘要
The yellow gene family plays a crucial role in insect pigmentation.It has potential for use as a visible marker gene in genetic manipulation and transgenic engineering in several model and non-model insects.Sadly,yellow genes have rarely been identified in Stratiomyidae species and the functions of yellow genes are relatively unknown.In the present study,we first manually annotated and curated 10 yellow genes in the black soldier fly(BSF),Hermetia illucens(Stratiomyidae).Then,the conserved amino acids in the major royal jelly proteins(MRJPs)domain,structural architecture and phylogenetic relationship of yellow genes in BSF were analyzed.We found that the BSF yellow-y,yellow-c and yellow-f genes are expressed at all developmental stages,especially in the prepupal stage.Using the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system,we successfully disrupted yellow-y,yellow-c and yellow-f in the BSF.Consequently,the mutation of yellow-y clearly resulted in a pale-yellow body color in prepupae,pupae and adults,instead of the typical black body color of the wild type.However,the mutation of yellow-c or yellow-f genes did not result in any change in color of the insects,when compared with the wild type.Our study indicates that the BSF yellow-y gene plays a role in body pigmentation,providing an optimal marker gene for the genetic manipulation of BSF.
基金
funded by a Talent Research Project of Anhui Agricultural University(rc342219)
by an Anhui Province Key R&D Project(202204c06020020).
