摘要
目的:研究持续光照对小鼠肠道菌群结构及其代谢物的影响。方法:采用不同光照干预小鼠,随机分为正常光照(light/dark,LD)组及24 h持续光照(light/light,LL)组,干预10周后,测量各组小鼠体重、空腹血糖、血清游离脂肪酸、血清三酰甘油及血清总胆固醇水平,收集新鲜粪便,采用16S rRNA测序技术研究持续光照对小鼠肠道菌群alpha多样性、结构、物种群落构成的影响,通过非靶向代谢组学检测粪便代谢产物变化,揭示肠道菌群差异代谢产物及代谢通路变化。结果:与LD组相比,LL组体重、空腹血糖和血脂水平明显增高(P<0.05)。在门分类水平,LL组厚壁菌门所占比例增高,拟杆菌门比例降低;从属水平分类,LL组拟杆菌、普雷沃氏菌_UCG-001丰度明显降低,而乳酸杆菌属、苏黎世杆菌属和Odo菌属丰度显著增加;非靶向代谢组学分析在正负离子模式下分析鉴定的粪便差异代谢物分别为65和73个,涉及6个主要相关代谢通路,分别为ABC转运蛋白、嘌呤代谢、嘧啶代谢、次级胆汁酸生物合成、蛋白质消化吸收和癌症中的胆碱代谢通路。结论:持续光照小鼠具有特异的肠道微生物群结构及其代谢产物。
AIM:To observe the effect of continuous light on the structure and differential metabolites of gut microbiota in mice.METHODS:The mice were randomly divided into normal light(light/dark,LD)group and 24-hour continuous light(light/light,LL)group.The body weight,fasting blood glucose,serum free fatty acids,serum triacylglycerol and serum total cholesterol levels of each group of mice were measured after 10 weeks.Fresh feces were collected,and 16S rRNA sequencing technology was used to study the effect of continuous light on the diversity,structure,and species composition of gut microbiota in mice.Additionally,liquid chromatography-mass spectrometry(LC-MS)analysis was performed to observe the effect of continuous light on the metabolites in mice.RESULTS:Compared with the LD group,the body weight,fasting blood glucose and lipid levels of the LL group were increased(P<0.05).At the phylum level,the proportion of Firmicutes in the LL group increased,while the proportion of Bacteroidetes decreased.At the class level,the abundance of norank_f_Muribaculaceae and Prevotellaceae_UCG-001 in the LL group decreased significantly,while the abundance of Lactobacillus,Turicibacter and Odoribacter increased significantly.Non-targeted metabolomics analysis identified 65 and 73 differential metabolites under positive and negative modes,involving six major metabolic pathways,including ABC transporters,purine metabolism,pyrimidine metabolism,secondary bile acid biosynthesis,protein digestion and absorption,and choline metabolism in cancer.CONCLUSION:The structure and metabolites of gut microbiota in mice exposed to continuous light are relatively specific,and inosine may be a key biomarker and potential therapeutic target for biological clock disorders.
作者
李燕
郑路
汤茹
魏健
石佩
李战
王有琴
LI Yan;ZHENG Lu;TANG Ru;WEI Jian;SHI Pei;LI Zhan;WANG Youqin(Department of Endocrinology,Heping Hospital Affiliated to Changzhi Medical College,Changzhi 046000,China;De-partment of Endocrinology,BenQ Hospital Affiliated to Nanjing Medical University,Nanjing 210000,China)
出处
《中国病理生理杂志》
北大核心
2025年第2期322-330,共9页
Chinese Journal of Pathophysiology
基金
山西省卫生健康委员会课题资助项目(No.2020140)
长治医学院附属和平医院院级课题资助项目(No.HPYJ202018)。
关键词
持续光照
生物钟
肠道菌群
continuous light
circadian clock
gut microbiota
