摘要
目的探讨白藜芦醇(RES)对口腔鳞状细胞癌(OSCC)细胞的增殖、迁移、细胞周期和糖酵解的影响及机制。方法用20、40、60、80、100、200、400μM的RES处理人OSCC细胞系HN6及Cal27,用CCK-8和细胞划痕实验分别评估RES处理对HN6和Cal27细胞增殖和迁移的影响;用流式细胞术检测细胞周期;用糖酵解试剂盒检测细胞培养液上清中葡萄糖,丙酮酸和乳酸的浓度;用RT-qPCR检测与增殖、迁移和细胞周期相关的mRNA表达水平。结果与对照组相比,RES处理组HN6和Cal27细胞存活率和迁移率均明显下降(P<0.05或P<0.01);RES处理组的细胞周期被阻滞于S期;RES处理组细胞中增殖相关基因MKi67和PCNA、S期细胞周期相关基因CyclinA2和CDK2以及迁移相关的基因MMP2、Twist和Snail2的mRNA表达水平均显著下降(P<0.01);此外,RES处理组培养液上清中葡萄糖浓度明显高于对照组(P<0.01),而丙酮酸和乳酸的浓度低于对照组(P<0.01)。结论RES的体外处理可显著抑制OSCC细胞的增殖、迁移和糖酵解代谢,将细胞周期阻滞在S期,并且诱导增殖、迁移及周期相关的基因表达下调。
Objective To investigate the effects of resveratrol(RES)on the proliferation,migration,cell cycle and glycolysis of oral squamous cell carcinoma(OSCC)cells and its underlying mechanisms.Methods Human OSCC cell lines HN6 and Cal27 were treated with 20,40,60,80,100,200 and 400μM RES,and the effects of RES treatment on the proliferation and migration of HN6 and Cal27 cells were evaluated by CCK-8 and cell scratch assay,respectively;cell cycle was detected by flow cytometry;the concentrations of glucose,pyruvic acid and lactic acid in the supernatant of the cell culture medium were detected by the glycolysis kit;the levels of mRNA expression associated with proliferation,migration and cell cycle were detected by RT-qPCR.Results Compared with the control group,the survival and migration rates of HN6 and Cal27 cells in the RES-treated group were significantly decreased(P<0.05 or P<0.01);the RES-treated group induced cell cycle arrest in S phase;in the RES-treated group,the mRNA expression levels of proliferation-related genes,MKi67 and PCNA,S-phase cell cycle-related genes,CyclinA2 and CDK2,and migration-related genes,MMP2,Twist and Snail2 were significantly decreased(P<0.01);the concentration of glucose in the supernatant of the culture medium of the RES-treated group was significantly higher than that of the control group(P<0.01),whereas the concentrations of pyruvate and lactate were lower than that of the control group(P<0.01).Conclusions This study suggests that in vitro treatment of resveratrol can significantly inhibit the proliferation,migration and glycolytic metabolism of OSCC cells,and induce cell cycle arrest in S phase and the down-regulation of proliferation,migration and cycle-related gene expression.
作者
成红玉
林鑫
韩泽文
徐振江
黄晓畅
CHENG Hong-yu;LIN Xin;HAN Ze-wen;XU Zhen-jiang;HUANG Xiao-chang(State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330006,China;Central Laboratory,Shenzhen Stomatology Hospital(Pingshan),Southern Medical University,Shenzhen 518118,China)
出处
《南昌大学学报（医学版）》
2024年第4期1-6,11,共7页
Journal of Nanchang University：Medical Sciences
基金
国家重点研发计划(2022YFA1304200)。
关键词
白藜芦醇
口腔鳞状细胞癌
增殖
迁移
细胞周期
糖酵解
resveratrol
oral squamous cell carcinoma
proliferation
migration
cell cycle
glycolysis
