摘要
目的:以人参皂苷为例探究内参物与待测成分浓度比对一测多评方法定量准确性的影响。方法:利用超高效液相色谱法(UPLC),采用外标法对人参中9种成分,人参皂苷Rg1、Re、Rf、Rh1、Rb1、Rc、Rb2、Rb3、Rd进行定量。以人参皂苷Rg1为内参物,采用一测多评法测定其余8种成分含量,并与外标法的定量结果比较,评估所建立的一测多评法的定量准确性。根据这9种人参皂苷的含量,用对照品配制人参皂苷Rg1与人参皂苷Rf、Rb2、Rd质量浓度比为100∶1、10∶1、5∶1、2∶1、1∶1、0.5∶1、0.25∶1的模拟样品溶液,验证内参物与其他成分的浓度差异对一测多评法定量准确性的影响。结果:一测多评法和外标法计算得到的人参样品中人参皂苷Re、Rf、Rb1、Rc、Rb2的含量结果一致[相对标准偏差(RSD)<3%],但2种方法计算得到的人参皂苷Rh1、Rb3、Rd的结果差异较大,RSD 7.06%~9.61%。经模拟样品溶液的验证,当内参物人参皂苷Rg1与待测成分浓度比为5或10甚至更高时,外标法和一测多评法计算结果差异较大。结论:定量成分与内参物浓度差异过大会增大一测多评法的定量误差,故建议在建立一测多评方法时,应选择与内参物浓度接近的成分进行定量。
Objective:To investigate the influence of concentration ratio(CR)between the internal reference and target components on the quantitative accuracy of quantitative analysis of multi-components by single marker(QAMS)by taking ginsenosides as an example.Method:Ultra performance liquid chromatography(UPLC)was employed,the contents of nine components in Ginseng Radix et Rhizoma(ginsenosides Rg1,Re,Rf,Rh1,Rb1,Rc,Rb2,Rb3,Rd)were determined by external standard method(ES).Using ginsenoside Rg1 as the internal reference,the contents of the remaining 8 ginsenosides were determined by QAMS,and the quantitative results were compared with those of ES to evaluate the quantitative accuracy of the established QAMS.According to the contents of these 9 ginsenosides,the simulated sample solutions with different CRs of ginsenoside Rg1 to ginsenosides Rf,Rb2,Rd were prepared with the reference substance(CR=100∶1,10∶1,5∶1,2∶1,1∶1,0.5∶1,0.25∶1),in order to validate the effect of the CRs between the internal reference and other components on the quantitative accuracy of the QAMS.Result:The results of ginsenosides Re,Rf,Rb1,Rc,Rb2 calculated by the two methods were the same with the relative standard deviation(RSD)<3%,however,the results of ginsenosides Rh1,Rb3 and Rd calculated by the two methods were different with RSDs of 7.06%-9.61%.According to the result of the simulated sample solution,the difference between the calculated results of ES and QAMS was large when the CR between the internal reference(ginsenoside Rg1)and other components was 5 or 10 or even higher.Conclusion:The quantitative error of QAMS will increase when the CR between the quantitative component and the internal reference is too large,so it is suggested that when establishing the QAMS,the components with close concentration to the internal reference should be selected for quantification.
作者
陈茜
孔沙沙
代云桃
CHEN Xi;KONG Shasha;DAI Yuntao(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2024年第13期184-191,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中国中医科学院科技创新工程项目(CI2023E001TS03,CI2023E001TS)。
关键词
多成分定量分析
一测多评法(QAMS)
浓度差异
人参皂苷
定量准确性
超高效液相色谱法(UPLC)
人参
multicomponent quantitative analysis
quantitative analysis of multi-components by single marker(QAMS)
concentration difference
ginsenoside
quantitative accuracy
ultra performance liquid chromatography(UPLC)
Ginseng Radix et Rhizoma
