摘要
目的研究芍药苷(PF)对脂多糖(LPS)诱导SH-SY5Y细胞损伤的保护及其作用机制。方法体外培养SH-SY5Y细胞,给予芍药苷预保护24 h,再通过LPS诱导SH-SY5Y细胞损伤,通过CCK-8法测定SH-SY5Y细胞的存活率;Western blot法检测NLRP、Bax、Bcl2蛋白的表达变化。结果芍药苷对LPS诱导SH-SY5Y细胞损伤具有不同程度的保护作用,细胞存活率提高;LPS组NLRP3及Bax蛋白表达明显增加,Bcl2蛋白表达减少,而不同剂量的芍药苷可以降低NLRP3及Bax蛋白的表达,增加Bcl2蛋白的表达。结论芍药苷对LPS诱导SH-SY5Y细胞损伤具有保护作用,保护作用可能与其降低炎症、抑制细胞凋亡有关。
Objective:To study the protective effect of paeoniflorin(PF)on the proliferation of SH-SY5Y(LPS)-induced neuroma cells(SH-SY5Y)and its mechanism.Methods:Lipopolysaccharide induced cell injury model was prepared.The morphological changes of SH-SY5Y cells were observed by microscope.Cell viability of SH-SY5Y cells was determined by CCK-8.The expressions of NLRP3,Bax and Bcl2 in each group of cells were analyzed by blot(Western).Results:Paeoniflorin had different protective effect on injury of SH-SY5Y cells;the expressions of NLRP3 and Bax in the LSP group increased significantly;different doses of LPS could decrease the expressions of NLRP3 and Bax and increase the expression of Bc12.Conclusion:Paeoniflorin has protective effects on LPS induced SH-SY5Y cell injury,which may be related to the reduction of inflammation related protein expression.
作者
宋歌
高伟
冀亚茹
李娜
郭婷婷
晏婷婷
徐晓燕
Song Ge;Gao Wei;Ji Yaru;Li Na;Guo Tingting;Yan Tingting;Xu Xiaoyan(Institute of Pharmacology,Shandong First Medical University&Shandong Academy of Medical Sciences,Taian 271016,China)
出处
《山东第一医科大学（山东省医学科学院）学报》
2021年第11期807-810,共4页
Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences
基金
国家级大学生创新创业训练计划(201710439140)。
