摘要
目的:探究Sox9用于治疗椎间盘退变的效果及调控机制。方法:将Ad-sox9和Ad-GFP各20μL分别转染至椎间盘退变兔的髓核组织中,转染后3、7、30、60天取材,采用免疫组化、免疫荧光和MRI等研究方法检测椎间盘髓核组织中II型胶原、蛋白多糖的表达情况,并分析对椎间盘退变的改善情况。结果:免疫组化染色显示sox9组中椎间盘髓核组织中II型胶原、蛋白多糖的表达明显升高,MRI显示sox9组椎间盘T2像信号有明显改善(P<0.05)。结论:体内转染腺病毒介导的sox9基因能够增加椎间盘内II型胶原和蛋白多糖的表达,并抑制椎间盘的退变进程。
Objective: To explore the regulatory mechanism of Sox9 in the treatment of intervertebral disc degeneration. Methods:Ad-sox9 and Ad-GFP 20 μL, respectively transfection nucleus pulposus of intervertebral disc degeneration of the rabbit, 3, 7, 30 and60 days after transfection materials, using the methods of immunohistochemical, fluorescence and magnetic resonance imaging(MRI)detection of intervertebral disc nucleus pulposus in the expression of type II collagen and proteoglycan, and analysis on the improvement of the intervertebral disc degeneration. Results: Immunohistochemical staining showed that the expression of type II collagen and proteoglycan in intervertebral disc nucleus pulposus tissues in sox9 group was significantly increased, and MRI showed that T2 image signal of intervertebral disc in sox9 group was significantly improved(P<0.05). Conclusion: In vivo transfected adeno-mediated sox9 gene can increase the expression of type II collagen and proteoglycan in intervertebral disc, and inhibit the degeneration process of intervertebral disc.
作者
赵哲
刘贾昆
王焕
王文波
徐宁
ZHAO Zhe;LIU Jia-kun;WANG Huan;WANG Wen-bo;XU Ning(Department of Orthopedics,First Affiliated Hospital of Harbin Medical University,Harbin,Heilongjiang,150001,China)
出处
《现代生物医学进展》
CAS
2020年第3期445-448,423,共5页
Progress in Modern Biomedicine
基金
黑龙江省教育厅科学技术研究项目(11551165)。
关键词
椎间盘
髓核
SOX9
Ⅱ型胶原
基因表达
Intervertebral disc
Nucleus pulposus
Sox9
Type II collagen
Gene expression
