摘要
目的 探讨陕西地区汉族T2DM患者中Calpain-10基因多态性与冠状动脉粥样硬化性心脏病(CHD)易感性的相关性.方法 选取2016年1月至2018年12月于宝鸡市人民医院行冠状动脉造影检查的T2DM患者669例,根据造影结果分为T2DM合并CHD组(CHD)415例和无CHD组(T2DM)254例.同期选取300名行冠状动脉造影检查排除CHD的非T2DM患者作为对照组(Con),采用聚合酶链反应-限制性内切酶分析法检测所有研究对象Calpain-10基因SNP43位点基因型.结果Con、T2DM组SNP43位点等位基因分布频率比较,差异有统计学意义(P<0.05);Con、CHD组SNP43位点基因型和等位基因分布频率比较,差异有统计学意义(P<0.05);CHD、T2DM组SNP43位点基因型和等位基因分布频率比较,差异有统计学意义(P<0.05).二元逐步回归分析结果显示,Calpain-10基因SNP43位点G等位基因是T2DM患者发生CHD的影响因素(OR 1.725,95%CI 1.027~2.899,P=0.007).结论 陕西地区汉族 T2DM 患者 Calpain-10 基因 SNP43 位点多态性可能与 CHD 易感性相关.
Objective To investigate the relationship between Calpain-10 gene polymorphism and coronary heart disease(CHD)in patients with type 2 diabetes mellitus(T2DM)in the Han population in Shaanxi region.Methods from our department during January 2016 to December 2018.All these patients were divided into two groups according to the results of coronary arteriography:T2DM with CHD(CHD group,n=415)and T2DM without CHD(T2DM group,n=254).Meanwhile,patients without T2DM and CHD according to the results of coronary arteriography were selected as controls(Con group,n=300).Genotype of Calpain-10 gene SNP43 was determined by polymerase chain reaction-restriction endonuclease enzyme analysis.Results The allele frequencies of SNP43 were significantly different between Con group and T2DM group(P<0.05).The genotype and allele frequencies of SNP43 were significantly different between Con group and CHD group(P<0.05).The genotype and allele frequencies of SNP43 were significantly different between CHD group and T2DM group(P<0.05).Binary stepwise regression analysis show that G allele of SNP43 was an independent risk factor for CHD in T2DM patients(OR 1.725,95%CI 1.027~2.899,P=0.007).Conclusion Calpain-10 gene SNP43 polymorphism may be related to CHD in T2DM patients in the Han population in Shaanxi province.
作者
魏欣辉
虎晓燕
WEI Xinhui;HU Xiaoyan(Department of Geriatrics,Baoji People’s Hospital,Baoji 721000,China)
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2020年第3期193-196,共4页
Chinese Journal of Diabetes
