摘要
目的:以虫草素(化合物A)为母核合成新化合物5'-呋喃甲酰酯-3'-脱氧腺苷(化合物B),并且进一步通过化合物B修饰纳米金得到了络合物C,研究二者体外抗肿瘤活性及抑菌活性。方法:利用1H NMR、13C NMR及MS对化合物B结构进行了表征确证,采用红外(IR)、紫外-可见分光光度计、X射线衍射(XRD)、透射电子显微镜(TEM)对络合物C结构及形貌进行了表征。结果:化合物B和络合物C的结构经过红外光谱、核磁表征,表明成功实现了目标产物的合成。通过MTT实验研究发现化合物A、化合物B和络合物C对HepG2细胞的增殖均有明显的抑制作用。抑菌实验结果表明,三者对大肠杆菌、枯草杆菌、金黄色葡萄球菌均有明显的抑菌效果。并且进一步对络合物C进行研究,发现其对大肠杆菌、枯草杆菌、金黄色葡萄球菌的最小抑菌浓度分别为30、7.5和30μg/mL。结论:本文的研究为新型抗肿瘤抗菌药物的研究提供一条新的思路。
Objective:A new compound of 5'-furanoyl ester-3'-deoxyadenosine(compound B)was prep synthesized with cordycepin(compound A)as the raw material,complex C of gold nanoparticles modified by compound B was prepared,and antitumor activity and bacteriostatic activity in vitro were studied.Methods:The structure of compound B was confirmed by 1H NMR,13C NMR and MS.The structure and morphology of complex C were characterized by infrared(IR),ultraviolet-visible spectrophotometer,X-ray diffraction(XRD),and transmission electron microscope(TEM).Results:The structures of compound B and complex C were characterized by IR and NMR,and synthesized successfully.MTT experiments showed that compound A,compound B,and complex C had significant inhibitory effects on the proliferation of HepG2 cells.The results of bacteriostatic experiment showed that all of them had significant bacteriostatic effects on E.coli,Bacillus subtilis,and Staphylococcus aureus.The antibacterial properties of complex C were investigated.The results showed that the minimum inhibitory concentrations of E.coli,B.subtilis,and S.aureus were 30,7.5 and 30μg/mL,respectively.Conclusion:The research of this paper provides a new idea for the research of new antitumor antibacterials.
作者
崔琳琳
王莹
雒江菡
袁甜
魏思亦
CUI Lin-lin;WANG Ying;LUO Jiang-han;YUAN Tian;WEI Si-yi(College of Pharmacy,Harbin University of Commerce,Harbin 150076,China;Heilongjiang Provincial Key Laboratory of Drug Prevention and Treatment for Senile Diseases,Harbin 150076,China)
出处
《食品工业科技》
CAS
北大核心
2020年第7期65-69,共5页
Science and Technology of Food Industry
基金
哈尔滨商业大学青年创新人才项目(17XN031)
哈尔滨商业大学大学生创新与创业培训项目(201710240020)。
关键词
虫草素
修饰
纳米金
活性
抗肿瘤
抑菌性
cordycepin
modification
gold nanoparticles
activity
anti-tumor
antibacterial activity
