摘要
为了测定翻黄合剂中芍药苷和盐酸小檗碱的含量。采用中性氧化铝柱对供试品溶液进行处理,使目标峰不被杂质峰干扰。采用YMC-PackProC18色谱柱(250×4.6mm),流动相为乙腈-0.15%磷酸溶液(梯度洗脱),流速为1.0mL/min,柱温为26℃,检测波长为245nm,建立了高效液相色谱测定方法。芍药苷和盐酸小檗碱的进样量分别在0.307~4.920μg(r=0.9996)和0.064~1.025μg(r=0.9998)范围内与峰面积呈良好的线性关系,平均回收率分别为91.10%(RSD=1.75%,n=6)和92.06%(RSD=1.69%,n=6)。该方法简单、可靠,可以同时检测翻黄合剂中芍药苷和盐酸小檗碱的含量,为翻黄合剂的质量控制提供了依据。
A method for the content determination of paeoniflorin and berberine hydrochloride in Fanhuang mixture. Neutral alumina column was used to treat the sample solution so that the target peak was not disturbed by the impurity peak. Used YMC-Pack Pro C18 analysis column( 250 mm×4.6 mm),mobile phase of acetonitrile-0. 15% phosphoric acid aqueous solution( gradient elution),the flow rate was 1. 0 m L/min,the column temperature was 26 ℃,the detection wavelength was 245 nm. The linear ranges of paeoniflorin and berberine hydrochloride were 0.307 ~ 4.920 μg( r = 0.9996) and 0.064 ~ 1.025 μg( r = 0.9998) respectively,and their average recovery rates were 91.10%( RSD = 1.75%,n = 6) and 92.06%( RSD = 1.69%,n = 6) respectively. The method is simple and reliable,and can simultaneously detect the content of paeoniflorin and berberine hydrochloride in Fanhuang mixture,providing a basis for the quality control of Fanhuang mixture.
作者
陆凤琪
周海嫔
陈文成
甘斌艳
王亚芳
LU Feng-qi;ZHOU Hai-pin;CHEN Wen-cheng;GAN Bin-yan;WANG Ya-fang(Anhui Muchuntang Natural Medicine Research and Development Co.,Ltd,Chuzhou,Anhui 239000,China;Chuzhou University,Chuzhou,Anhui 239000,China;Beijing Institute of Veterinary Drugs Control,Beijing 102629,China)
出处
《中国兽药杂志》
2019年第7期25-29,共5页
Chinese Journal of Veterinary Drug
基金
滁州市农业与社会发展领域科技计划项目(201711)
关键词
翻黄合剂
芍药苷
盐酸小檗碱
HPLC
Fanhuang mixture
paeoniflorin
berberine hydrochloride
HPLC
