摘要
目的:探究转录因子TWIST2和PPARγ在非酒精性脂肪性肝病患者血清以及正常人肝细胞株LO2脂肪变模型中的表达变化,为非酒精性脂肪性肝病的诊断或治疗提供新线索。方法:收集2017年12月-2018年4月在山东省千佛山医院体检中心进行健康查体的、影像学诊断为非酒精性脂肪性肝病的患者共计393例血清标本,采用ELISA方法分析血清中TWIST2和PPARγ的表达,并研究其与非酒精性脂肪性肝病发展的关系;使用油酸诱导建立肝细胞脂肪变模型,采用Western Blot技术检测TWIST2和PPARγ在模型细胞中的套达变化,研究其与肝细胞脂肪变的关系。结果:非酒精性脂肪性肝病各组患者血清中的TWIST2浓度均较健康对照组降低,差异有统计学意义(P<0.05);血清中的TWIST2和PPARγ的相关性分析,Pearson相关系数为0.812;转录因子TWIST2在肝细胞脂肪变模型中表达下调2349%,差异有统计学意义(P<0.05).结论:转录因子TWIST2在肥胖所致的非酒精性脂肪性肝病的发病过程中表达下降;血清中转录因子TWIST2与PPARy表达存在正向相关性.
Objective: To investigate the expression changes of transcription factors TWIST2 and PPARγ in the scrum of patients with non -alcoholic fatty liver disease as well as steatosis model of the normal human hepatocyte line LO2, providing new clues for the diagnosis or treatment of non-alcoholic fiitty liver disease. Methods: A total of 393 serum samples were collected from patients who were diagnosed as non-alcoholic fatty liver disease in the physical examination center of Shandong Provincial Qianfoshan Hospital from 2017.12 to 2018.04. The expression of TWIST2 and PPARγ in sennn was analyzed by ELISA. Hepatocyte steatosis model was induced by oleic acid, and the expression changes of TWIST2 and PPARγ in model cells were detected by Western Blot. Results: The concentration of TWIST2 in semin of patients with non -alcoholic fatty liver disease was lower than that of healthy control. The correlation analysis of TWIST2 and PPARγ in sennn showed that the Pearsoil correlation coefficient was 0.812. The transcription factor TWIST2 is downregulated in the hepatocyte steatosis model by 23.49%. Con elusion: Transcription factor TWIST2 is downregulated during the pathogenesis of non-alcoholic fiitty liver disease. There is a positive correlation between the expression of transcription factor TWIST2 and PPARγ in serum.
作者
张艳美
逮素梅
葛肖肖
马万山
ZHANG Yan-inei;LU Su-mei;GE Xiao-xiao(Department of Laboratory Medicine, Shandong Provincial Qianfoshan Hospital,Shandong Jinan 250014)
出处
《医学检验与临床》
2019年第3期9-12,共4页
Medical Laboratory Science and Clinics
基金
山东省重点研发项目(2017G006024).
