摘要
目的 :建立复张性肺水肿实验模型。方法 :在麻醉下将带阀门的 12F胸管放入家兔右侧胸腔 ,麻醉清醒后通过阀门打气至右肺完全萎陷 ,此后每天经阀门补充一定量气体以维持肺萎陷状态 ,7d后抽气 ,建立实验模型。结果 :抽气后 5h肺湿干比为 5 .0 9± 0 .2 1。CT检查见右肺密度增高 ,抽气后 3～ 5h更明显 ;病理形态学观察见 :肺泡水肿、渗出 ,中性粒细胞浸润 ,Ⅱ型肺泡上皮细胞出现核周裂隙 ,胞质中内质网及线粒体肿胀、板层小体排空现象明显。结论 :我们建立的动物模型 ,经济易行且重复性好 ,是一个较为理想的动物模型。
bjective:This experiment is designed to establish a model of reexpansion pulmonary edema. Method:This experiment is designed by application of negative pressure to a No 12 French chest tube which was inserted through a right midinstercostal space under the rabbit anesthetized before 7days. Collapse of the right lung was induced by injection of air into the right pleural cavity, and injections were then repeated daily to maintain a state of nearly total collapse for 7 days. Result:Five hours after the reinflation, the wet to dry ratio is 5.09±0.21.Thoracic CT scan can recognize edema.Histologic section revealed edema of alveolar space and interstial edema. Electron microscopy found the notable change of type Ⅱalveolar endothelium. Conclusion:Our model of reexpansion pulmonary edema appears to be an ideal one because it is economic, feasible and can replicate many clinical features of this kind of edema.
出处
《江苏大学学报（医学版）》
CAS
2002年第4期339-341,共3页
Journal of Jiangsu University：Medicine Edition
