摘要
目的 :探索脊髓灰质炎 (脊灰 )流行与野毒株性状的关系。方法 :选择了 1991年安岳县和 1992年会东县各一例临床诊断为脊灰的患者 ,从其粪便标本中各分离出一株病毒 ,用微量中和试验进行血清学鉴定 ,PCR— RFL P法进行型内鉴定 ,对 VP1区 30 0个核苷酸进行测序。结果 :血清学定型均为脊灰 型病毒 ,经 PCR— RFL P法检测 ,判为野病毒。核酸序列分析证实两株病毒与 Sabin 有明显差异为野病毒。两毒株间核酸序列不完全一致 ,有 2 0个核苷酸的差异。结论 :不同年份、不同地方流行株间的序列有差异。四川省脊灰流行与毒株间核酸序列的差异有关。但我国现行疫苗 OPV能有效预防我省脊灰 。
Objective:To explore the relationship between poliomyelitis epidemics and wildvirus characteristics.Methods:Two patients were clinically diagnosed as poliomyelitis,one in Anyue County,1991 and one in Huidong County,1992.Two virus strains were isolated in the stool samples,and the two isolates were detected by microtitration neutralization,Polymerase Chain Reaction Restriction Fragment Length Polymorphism(PCR-RFLP),and neucleotide sequence analysis.Results:They were all type Ⅰ polioviruses by microtitration neutralization,and interpreted as wildviruses by PCR-RFLP.Nucleotide sequence analysis were performed and they showed significant differences from Sabin Ⅰ,they were confirmed as polio wildviruses.There were 20 neucleotide differences in sequences between the two isolates.Conclusions:In different places and times,there were differences in the sequences of epidemic strains.The outbreak of polio in Sichuan was related to difference of nucleotide sequence in strains,but current use of trivalent oral polio vaccine could effectively prevent the infection of poliovirus.Routine immunization and national immunization day activities were powerful measures to prevent and eradicate poliomyelitis.
出处
《现代预防医学》
CAS
2000年第3期340-341,共2页
Modern Preventive Medicine
关键词
脊灰Ⅰ型野病毒
序列分析
流行株
PCR-RFLP
Wild type Ⅰ poliovirus
Nucleotides sequence
Polymerase Chain Reaction-Restriction Fragment Length Polymorphism assay
