摘要
目的研究金银花(忍冬Lonicera japonica干燥花蕾)的化学成分。方法反复利用硅胶柱色谱、Sephadex LH-20、中压柱色谱及制备液相色谱等方法分离纯化;通过MS、1H-NMR及13C-NMR等技术鉴定化合物结构。结果从金银花醋酸乙酯部位分离得到12个化合物,分别鉴定为对羟基苯甲酸(1)、香草酸(2)、槲皮素-3-O-α-L-吡喃鼠李糖苷(3)、山柰酚-3-O-β-D-吡喃葡萄糖苷(4)、3′,4′,7-三羟基-3,5-二甲氧基黄酮(5)、异鼠李素(6)、金丝桃苷(7)、槲皮素-7-O-β-D-吡喃葡萄糖苷(8)、环裂马钱子酸(9)、断氧马钱子苷半缩醛内酯(10)、5-O-咖啡酰奎宁酸甲酯(11)、豆甾醇(12)。结论化合物5首次从忍冬属植物中分离得到;化合物1~4、6、11、12首次从金银花中分离得到。
Objective To investigate the chemical constituents of the flower buds of Lonicera japonica.Methods The chemical constituents were isolated by repeated silica gel chromatography,Sephadex LH-20,medium pressure column chromatography and preparative HPLC,and their structures were elucidated by spectroscopic analyses and comparison of MS,1H-NMR and 13C-NMR data with those reported in literature.Results Twelve compounds from the Et OAc fraction of L.japonica included p-hydroxybenzoic acid(1),vanillic acid(2),quercetin-3-O-α-L-rhamnopyranoside(3),kaemnpferol-3-O-β-D-glucopyranoside(4),3′,4′,7-trihydroxy-3,5-dimethoxyflavone(5),isorhamnetin(6),hyperoside(7),quercetin-7-O-β-D-glucopyranoside(8),secologanic acid(9),vogeloside(10),5-O-caffeoylquinic acidmethylester(11)and stigmasterol(12).Conclusion Compound 5 is obtained from the plant in Lonicera Linn.for the first time;Compounds 1—4,6,11 and 12 are obtained from this plants for the first time.
作者
倪付勇
温建辉
李明
赵祎武
王振中
萧伟
NI Fu-yong;WEN Jian-hui;LI Ming;ZHAO Yi-wu;WANG Zhen-zhong;XIAO Wei(The Key Laboratory for the New Technique Research of TCM Extraction and Purification,State Key Laboratory of New-Tech for Chinese Medicine Pharmaceutical Process,Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China)
出处
《中草药》
CAS
CSCD
北大核心
2017年第18期3689-3692,共4页
Chinese Traditional and Herbal Drugs
基金
科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203)
