摘要
通过体外培养兔原代肾小管上皮细胞,来研究大血藤、吴茱萸、豨莶草及川牛膝4种中药材提取物对肾小管上皮细胞增殖作用的影响。利用MTT法测定大血藤、吴茱萸、豨莶草及川牛膝4种中药材的水提与醇提的3mg/L,30 mg/L和300 mg/L三个浓度组对兔原代肾小管上皮细胞增殖作用的影响。结果表明,吴茱萸和豨莶草的醇提300 mg/L浓度组及大血藤的水提300 mg/L浓度组对兔原代肾小管上皮细胞的增殖作用抑制很强,其存活率分别仅有24.12%,16.03%和30.84%,而大血藤的醇提3 mg/L浓度组和豨莶草的醇提30 mg/L及3 mg/L浓度组对兔原代肾小管上皮细胞的增殖作用具有促进作用,其存活率达到了107.91%,114.95%和111.45%。大血藤、吴茱萸、豨莶草及川牛膝4种中药材对兔原代肾小管上皮细胞增殖作用均有不同程度的影响。
The effects of extracts of four Chinese herbal medicines, namely Sargentodoxa cuneata,Tetradium rutiearpum,Siegesbeekia orientalisL and Cyathula offieinalis Kuan, on the proliferation of renal tubular epithelial cells were studied by culturing rabbit primary renal tubular epithelial cells in vitro. The effects of 3 mg/L, 30 mg / L and 300 mg / L of water extraction and alcohol extraction of the four herbal medicines on tubule epithelial cell proliferation were studied by MTT method. The results showed that 300 mg / L of the alcohol extract of T. ruticarpum and S. orientalis and the water extract of 300 mg / L of S. cuneata significantly inhibited the proliferation of primary renal tubular epithelial cells by 24. 04%, 16. 03% and 30. 84%, respectively. 3 mg / L of the alcohol extract of S. cuneata and 30 mg / L and 3 mg / L of alcohol extract of S. orientalis showed a proliferation effect on primary renal tubular epithelial ceils, and the survival rate reached 107.91% , 114.95% and 111.45%. All of the four Chinese herbal medicines had some effects on the proliferation of primary renal tubular epithelial cells in rabbit.
出处
《山地农业生物学报》
2017年第4期76-79,共4页
Journal of Mountain Agriculture and Biology
基金
贵州省药食同源资源研究开发科技创新人才团队(黔科合人才团队(2015)4010号)
贵州省中药民族药制药工程专业学位研究生工作站(黔教研合JYSZ字[2014]002)
贵州省科技重大专项子课题(黔科合J重大字[2015]2002-3-4)
贵州省高层次创新型人才培养(黔科合人才(2015)4032号)
关键词
大血藤
吴茱萸
豨莶草
川牛膝
增殖作用
sargentodoxa cuneata
tetradium ruticarpum
siegesbeckia orientalisL
cyathula officinalis kuan
proliferation effect
