摘要
目的对我院胃癌和结直肠癌等消化道肿瘤常用治疗药物的相关单核苷酸多态性(single nucleotide polymorphisms,SNPs)的特点进行分析。方法抽取消化道肿瘤患者静脉血,采用荧光染色原位杂交检测法对药物相关基因位点进行检测。应用Excel2013对原始数据进行统计分析,并采用SPSS13.0统计软件对每一基因位点的基因型分布进行χ2检验,判断等位基因频率分布是否符合Hardy-Weinberg平衡定律。结果 242例患者共检测了涉及14个药物相关基因的1 743个位点。除二氢嘧啶脱氢酶基因(DPYD)、亚甲基四氢叶酸还原酶基因(MTHFR)、细胞色素P4501B1基因(CYP1B1)外,其他基因位点的基因型分布均符合Hardy-Weinberg平衡定律。结论中国消化道肿瘤患者的药物相关基因单核苷酸多态性的分布特点,因地域和种族的不同,表现出自身的分布特点,应该以其基因多态性进行精准抗肿瘤药物治疗。
OBJECTIVE To analyze of the characteristics of single nucleotide polymorphisms of the commonantineoplastics in the treatment of gastric cancer and colorectal cancer in our hospital. METHODS Venous blood was collected from patients with digestive tract tumor, and the drug related gone loci were detected by fluorescence staining in situ hybridization. The original data are stored in Microsoft Excel to establish a database for routine statistics and chi square test was conducted to determine whether the distr^butlon of allele frequency distribution is in accordance with the law of Hardy Weinberg. RESULTS A Total of 1 743 loci of 14 drug related genes were detected in 242 patients. The loci genotype distribution were in accordance witb Hardy Weinberg equilibrium except di- hydrotestosterone dihydropyrimidine dehydrogenase gene（ DPYD ）, methylenetetrahydrofolate reductase gene（MTHFR） and cyto- chrome P4501 B1 gone （ CYP1 B1 ）. CONCLUSION The patients with digestive tract tumor in China have their own characteristics in single nucleotide polymorphisms of drug related genes. We should take this as the basis for accurate anti-tumor drug treatment.
作者
刘颖
马旭
吴海伟
张艳华
LIU Ying MA Xu WU Hai-wei ZHANG Yan-hua(Key Laboratory of Carcinogenesis and Translational Research （Ministry of Edueation/ Beijing ） , Department of Pharmacy, Peking University Cancer Hospital ＆ Institute, Beijing 100142, China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2016年第24期2124-2128,共5页
Chinese Pharmaceutical Journal
关键词
消化道肿瘤
药物基因组学
单核苷酸多态性
gastrointestinal neoplasm
pharmacogenomics
single nueleotide polymorphism
