摘要
选择适用于婴幼儿配方粉的3株双歧杆菌作为研究菌株,通过设计不同的平板类型和培养方式来验证适用于以上3株双歧杆菌的最优计数方式。结果:双层平板(下层为20 mL MRS,上层为10 mL石蜡)可以无需厌氧培养箱即可实现对含动物双歧杆菌Bb-12、乳双歧杆菌Bi-07或乳双歧杆菌HN019产品的可靠计数,而且检测结果与厌氧培养箱法无显著差异。
Three Bifidobacterium strains(used for infant formula powder)were chose as subject strains.Then the optimal methods were studied by different plate types and culture methods for viable cell count.Results:reliable enumeration could be realized by double layer plate(lower for 20 mL MILS,upper layer was 10 mL paraffin)without anaerobic incubator for Bifidobactetium animalis Bb-12,Bifidobacterium lactis Bi-07 and Bifidobacterium lactis HN019.And no significant difference were found between the test method and anaerobic incubator method.
作者
贾宏信
苏米亚
陈文亮
齐晓彦
JIA Hong–xin;SU Mi–ya;CHEN Wen–liang;QI Xiao–yan(State Key Laboratory of Dairy Biotechnology,Shanghai Engineering Research Center of Dairy Biotechnology,Dairy Research Institute,Bright Dairy&Food Co.Ltd.,Shanghai 200436,China)
出处
《中国乳品工业》
CAS
CSCD
北大核心
2016年第10期11-14,共4页
China Dairy Industry
基金
上海市经委引进技术的吸收与创新计划(14XI-1-15)
上海市科学技术委员会工程中心能力提升项目(16DZ2280600)
关键词
婴幼儿配方粉
双歧杆菌
计数
双层平板
infant formula
Bifidobacterium
enumeration
double layer plate
