摘要
以大白菜叶片为材料,研究了过氧化氢酶对吡虫啉和毒死蜱两种杀虫剂残留的降解效果。在单因素试验基础上,采用响应面法对杀虫剂残留降解的反应条件进行优化。结果表明,降解杀虫剂残留最佳工艺条件为:pH7、过氧化氢酶质量浓度40 mg·L^(-1)、反应温度35℃、反应时间15 min,毒死蜱和吡虫啉降解率分别为82.05%和80.29%。处理后大白菜叶片毒死蜱和吡虫啉含量均达到食品中农药最大残留限量的食品安全国家标准。利用过氧化氢酶降解食品中农药残留,可以降低食品安全风险,具有较好应用前景。
The degradation rate of pesticide residues from imidacloprid and chlorpyrifos in Chinese cabbage leaves were analyzed by leaching methods such as catalase.Based on the single factor experiment,the reaction condition of degradation from pesticide residues was optimized by using response surface method.The results showed that the optimum conditions were as followings:pH was 7,catalase mass concentration was 40 mg·L^-1,the reaction temperature was 35℃,and the reaction time was 15 min.Then the degradation rate could reach 82.05%and 80.29%for imidacloprid and chlorpyrifos,respectively.The national food safety standard of maximum residue limits for pesticides in food was reached in tea leaves of imidacloprid and chlorpyrifos content.It also showed that food safety risk reduction by enzymes had great application prospect in leaching bioremediation of pesticide residues in food.
作者
林维晟
施小聪
廖素兰
蔡晨慧
Lin Wei-sheng;Shi Xiao-cong;Liao Su-an;Cai Chen-hui(Fujian Provincial Key Laboratory of Eco-industrial Green Technology,Ecology and Resources Engineering College,Wuyi University,Wuyishan 354300)
出处
《食品工业》
CAS
北大核心
2016年第9期154-159,共6页
The Food Industry
基金
国家自然科学基金--青年基金项目(21407118)
福建省科技厅引导(重点)项目(2015N0035)
福建省科技厅高校专项项目(JK2011058)
南平市科技局科技计划项目(N2012Z06(4))
大学生创新训练计划建设项目(教育部)(201410397009)
关键词
过氧化氢酶
降解
杀虫剂
响应面法
蔬菜
catalase
degradation
pesticide
response surface method
vegetable
