摘要
目的建立自动化微生物比浊法测定血清环丙沙星(CPLX)浓度。方法以标准血清配制系列浓度CPLX与107cfu/ml标准菌ATCC27853反应 ,在自动化微生物分析仪中测定其吸收度。结果在8～0.0625μg/ml浓度范围内 ,浓度与吸收度呈线性关系(r=0.9950,n=8) ,回归方程为C=9.4020 -22.55A ,最低检测浓度为0.03μg/ml。方法回归率为96 5 %～108.0 %,日内变异系数为2.11 %～9.13 %,日间变异系数为1.99 %～9.72 %。结论该方法为一快速、方便、精确的测定CPLX的方法。
Aim The application of microbiological turbidimetric method to determination of serum concentrations (C) of ciprofloxacin (CPLX) was studied.Methods A series diluted concentrations of CPLX were prepared with blank serum. The solution was put in a research unit with standard bacteria (ATCC 27853) dilution of 107 cfu/ml and incubated in Avantage analysis system. Absorbance (A) was recorded once every 5 min in a wavelength of 670 nm.Results There was a liner relationship (r=0.9950,n=8) between concentrations of CPLX and absorbance. The regression equation was C=9.4020-22.55 A. The lowest detectable concentration was 0.03 μg/ml. The rate of recovery of the method was 96.5%～108.0%. The coefficient of variation within a day was 2.11%～9.13% while the coefficient of variation in different days was 1.99%～9.72%. Conclusion The microbiological turbidimetry is a rapid, convenient and accurate method to determine the concentration of CPLX.
