摘要
目的探讨长链非编码RNA脑细胞质RNA1（Brain Cytoplasmic RNA 1,BCYRN1）在食管鳞状细胞癌（Esophageal Squamous Cell Carcinoma,ESCC）中的表达及其预后意义。方法应用实时荧光定量PCR检测70例接受根治术治疗的ESCC患者癌组织及对应癌旁组织中BCYRN1的表达水平,并分析BCYRN1相对表达水平与ESCC患者临床病理特征及预后的关系。结果 1与癌旁组织相比,BCYRN1在57.1%（40/70）的ESCC癌组织中表达上调,且ESCC癌组织中BCYRN1表达水平明显增高（t=2.325,P=0.023）。2BCYRN1的相对表达水平与患者临床病理因素如年龄、临床病理分期及组织学分级等均无关。3Kaplan-Meier生存分析显示BCYRN1高表达组患者的疾病无进展时间（Disease Free Survival,DFS）及总生存时间（Overall Survival,OS）显著缩短（χ2=4.488,P=0.034和χ2=4.629,P=0.031）。多因素回归分析临床病理特征及BCYRN1表达水平对DFS和OS的影响结果显示,BCYRN1表达水平对DFS和OS的风险比（HR）分别为2.165（95%CI：1.119-4.186,P=0.022）和2.242（95%CI：1.120~4.488,P=0.023）。结论 BCYRN1高表达与预后差有关,可能是接受根治术治疗的ESCC患者的预后指标。
Objective To explore the expression and prognosis significance of long noncoding RNA Brain Cytoplasmic RNA 1（ BCYRN1） in esophageal squamous cell carcinoma（ ESCC） patients who received radical resection. Methods Quantitative real- time polymerase chain reaction（ qRT- PCR） was used to detect the expression level of BCYRN1 in cancer tissue and paired adjacent normal tissue samples from 70 ESCC patients who received radical surgical resection.The correlation of the relative expression level of BCYRN1 with clinical- pathological features and prognosis were also analyzed. Results 1 BCYRN1 was upregulated in 57. 1%（ 40 /70） ESCC tissues and the relative expression of BCYRN1 was significantly higher in ESCC tissues compared with adjacent normal tissue samples（ t = 2. 325,P =0. 023）. 2The high levels of BCYRN1 expression were not related to clinical- pathological features such as age,TNM stages,histological grade. 3 Kaplan- Meier analysis showed high expression levels of BCYRN1 were correlated with poor prognosis in ESCC patients. Patients with a high level of BCYRN1 had a shorter DFS and OS than those with low BCYRN1 expression（ χ2= 4. 488,P = 0. 034 and χ2= 4. 629,P = 0. 031）. ON multivariate analysis,the HR of BCYRN1 expression was 2. 165（ 95% CI： 1. 119 ~ 4. 186,P = 0. 022） for DFS and 2. 242（ 95% CI： 1. 120 ~ 4. 488,P =0. 023） for OS. Conclusion Our results indicate that high expression of BCYRN1 reflect poor prognosis and might be a predictive marker for ESCC patients who received radical resection.
出处
《医药论坛杂志》
2016年第2期4-8,12,共6页
Journal of Medical Forum
基金
国家自然科学基金面上资助项目(81472714)
