摘要
研制了一套基于Linnik干涉仪的全场光学相干层析(FF-OCT)系统对细胞进行层析成像。不同于其他FF-OCT系统的成像方法,该系统采用了基于Hilbert变换的图像恢复算法,只需2幅移相干涉图就可以恢复样品的结构信息,即使移相出现误差,仍能得到样品层析图。系统的实测纵向、横向分辨率分别达到1.1μm、2.5μm。通过对洋葱表皮细胞的初步成像实验,验证了该FF-OCT系统的可行性,为进一步实现高分辨率细胞成像奠定了基础。
A Linnik type full-field optical coherence tomography （FF-OCT） system is developed for to- mographic imaging of biological cells. Different from other imaging methods used in FF-OCT systems, an image restoration method based on Hilbert transform is adopted, which requires only two interference images to recover the structure information of the sample. Even if the phase shift error occurs, the sample chromatogram can still be obtained. The measured longitudinal and lateral resolutions of the system reach 1.1 μm and 2.5 μm, respectively. Feasibility of the FF-OCT system is verified by preliminary imaging ex- periments of onion epidermal cells, and it lays foundation for the further realization of high resolution cell imaging.
出处
《量子电子学报》
CAS
CSCD
北大核心
2016年第4期437-443,共7页
Chinese Journal of Quantum Electronics
基金
国家自然科学基金青年基金
11402037
常州市呼吸医学工程重点实验室项目
CM20133005~~
关键词
图像处理
显微
全场光学相干层析
成像系统
希尔伯特变换
image processing
microscopy
full-field optical coherence tomography
imaging system
Hilberttransform
