摘要
目的研究母爱剥夺（MO）对缺氧缺血性脑损伤（HIBD）新生大鼠远期学习记忆、海马病理学及巢蛋白表达的影响。方法按Rice法制备7日龄sD雄性HIBD模型，按随机数字表法随机分为MD组和对照组。另设假手术组。MD组于造模后第2天开始母子分离至出生第21天，28d后应用Morris水迷宫实验测试大鼠学习记忆能力，HE染色观察海马病理形态学变化，免疫组织化学方法检测海马巢蛋白nestin的表达。结果体质量结果显示，假手术组体质量高于对照组和MD组，对照组高于MD组，差异有统计学意义（q=9．8608、3．8807、5．9801，P均〈0．05）。水迷宫实验结果显示，MD组定位航行试验和空间探索试验成绩差于对照组和假手术组，假手术组成绩优于对照组，差异有统计学意义（P均〈0．05）。HE染色显示，假手术组右侧海马神经元排列紧密整齐；对照组右侧海马神经元排列相对整齐，但细胞层数减少；MD组右侧海马神经元弥散性空泡变性，细胞排列疏松、紊乱。免疫组织化学结果显示，MD组右侧海马nestin阳性细胞数明显少于对照组，假手术组nestin阳性细胞数少于MD组，组间比较差异有统计学意义（P均〈0．05）。结论MD加重了HIBD大鼠学习记忆能力的损害，且使其海马nestin阳性细胞数减少，不利于脑损伤的修复。
Objective To observe the effect of maternal deprivation（MD） on learning and memory ability and hippocampal pathology and nestin expression in rats with hypoxic - ischemic brain damage （HIBD）. Methods The models of HIBD SD male rats were established by the method of Rice, and were randomly divided into 2 groups ： MD group and control group. In addition,the shamoperation group（ sham group） models were established. The MD group rats were separated from their mother 3 hours per day from the second day after modeling to the 21^st postnatal day. After 28 postnatal days, Morris water maze was used to evaluate the learning and memory ability of the rat models. HE staining was employed to observe the hippocampal pathological change in the rats. Then, the expression of nestin in the hippocampus was measured by the method of immunohistochemistry. Results Their body mass changes showed that quality of sham group was higher than that of the control and the MD groups, and quality was improved in the control group, compared with the MD group, and the differences were statistically significant（ q = 9. 860 8,3. 880 7,5. 980 1, all P 〈 0.05）. The water maze scores of the MD group in place navigation test and spatial probe test were much lower than that of the control group and the sham group, and the scores of the sham group were higher than that of the control group, the differences were statistically significant （ all P 〈 0.05 ）. The findings of HE stain showed that the pathology in the right - sided hippocampus of the sham group was normal and neurons were well - arranged, and that of control group was minimally abnormal, and the neurons were almost arranged orderly and remained normal. While, the pathomorphology of the MD group was obviously abnormal, the neurons were arranged disorderly, many of the neurons lost. According to the immunohistochemical findings, the number of nestin - positive ceils in right - sided hippocampus of the MD group was significantly less than that of the control group, and the number of nestin - positive cells of the sham group was less than that of the MD group,which showed significant differences among the groups （ all P 〈 0.05 ）. Conclusions MD aggravated injury to learning and memory ability of neonatal rats with HIBD, and decrease the number of nestin - positive cells of MD markedly, which is not good for the recovery of brain injury.
出处
《中华实用儿科临床杂志》
CSCD
北大核心
2016年第2期146-149,共4页
Chinese Journal of Applied Clinical Pediatrics
基金
四川省教育厅重点科研项目（13ZA0210）
关键词
缺氧缺血
母爱剥夺
学习记忆
病理学
巢蛋白
Hypoxic - ischemic
Maternal deprivation
Learning and memory
Pathology
Nestin
