摘要
目的筛查恒河猴BST-2基因编码区单核苷酸多态性(coding-region single nucleotide polymorphism,c SNP),研究其对蛋白结构和功能的影响。方法提取恒河猴外周血RNA,RT-PCR扩增,单克隆测序比对,确定猴BST-2的c SNP位点;通过蛋白质结构分析软件对蛋白结构进行分析;比较不同基因型猴体内SHIVSF162 p 3复制水平的差异。结果序列比对发现8个非同义突变位点;经Psipred软件预测,其中G41A、T128C、C129和A333C c SNP位点可影响BST-2蛋白二级结构。在SHIVSF162 p 3感染平台期,参考基因型猴(DLQ)病毒复制水平要高于GLQ型猴(P<0.05),其他基因型猴之间无显著差异。结论c SNP(G41A)可能影响BST-2的抗病毒功能,这为后续研究提供参考信息。
Objective To explore the impacts of cSNP on the structure and function of rhesus macaque’s BST-2.Methods Extracting RNA from peripheral blood of rhesus macaques,then RT-PCR,Monoclonal sequencing to find the cSNP sites;Forecasting the structure of these proteins;Comparing the Level of SIVmac239 replication between the different genotypic Rhesus macaques.Results We found 8 non-synonymous mutation sites,in the 8 non-synonymous mutation sites,Only G41A,T128C,C129 and A333C change the secondary protein structure of BST-2 by forecasting of Psipred software;At the plateau of SHIVSF162p3 replication,The SHIVSF162p3 replication level of reference genotype rhesus macaque(DLQ)is higher than rhesus macaques of GLQ genotype,other genotypes rhesus macaques have no significant difference.Conclusion cSNP(G41A)may influence the antiviral activity of rhesus macaque’s BST-2,this study gives us a reference to further research work.
作者
董志会
王卫
丛喆
熊圣文
骆杨
陈霆
魏强
DONG Zhi-hui;WANG Wei;CONG Zhe;XIONG Sheng-wen;LUO Yang;CHENG Ting;WEI Qiang(Comparative Medicine Center,Peking Union Medical College(PUMC)&Institute of Medical Laboratory Animal Science,Chinese Academy of Medical Sciences(CAMS),Key Laboratory of Human Diseases Comparative Medicine,Ministry of Health,Key Laboratory of Human Diseases Animal Models,State administration of Traditional Chinese medicine,Beijing 100021,China)
出处
《中国比较医学杂志》
北大核心
2015年第7期20-24,共5页
Chinese Journal of Comparative Medicine
基金
“十二五”传染病重大专项课题(2012ZX10004-501-001,2013ZX10004-608-003)
