摘要
建立了一种在保健食品中检测西地那非的直接竞争酶联免疫吸附分析法(dc ELISA)。经优化最佳反应条件为:包被浓度为100ng/m L,酶标抗体稀释倍数为70倍,标准品稀释液为p H=9,0.01mol/L PBST溶液,抗体竞争反应50min。本方法的半抑制浓度IC_(50)为1.47ng/m L,线性范围(IC_(20)~IC_(80))为0.12~17.65ng/m L,检测限IC_(15)为0.0845ng/m L,批内批间变异系数均小于20%,保健食品补肾糖浆的添加回收率在86.0%~111.0%之间,满足实际样品的快速筛检需求。
A direct competitive enzyme-linked immunoassay(dcELISA)was developed for determination of sildenafil.The results showed that the optimized parameters were as follows:100ng/mL for coating antigen concentration,70 times dilution of enzyme conjugated antibody,dilution solution of sildenafil in 0.01mol/L PBST(pH=9)and reaction time for 50min.This developed method presented an IC_(50) of 1.47ng/mL,a linear range(IC_(20)-IC_(80))of 0.12-17.65ng/mL,a detection limit IC_(15) of 0.0845ng/mL.Intra-and inter-batch relative standard deviations(RSD)were below 20%.The recoveries of sildenafil in spiked samples ranged from 86.0%-111.0%.In conclusion,the dcELISA was a practical method for trace detection of sildenafil in real samples.
作者
李瑞婷
陆建超
王弘
杨金易
徐振林
孙远明
沈玉栋
LI Rui-ting;LU Jian-chao;WANG Hong;YANG Jin-yi;XU Zhen-in;SUN Yuang-ming;SHEN Yu-dong(College of Food Science,South China Agricultural University,Guangdong Provincial Key Laboratory of Food Quality and Safety,Laboratory of Quality and Safety Risk Assessment in Agricultural Products Preservation of Ministry of Agriculture of P.R.China,Guangzhou 510642,China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第24期84-88,95,共6页
Science and Technology of Food Industry
基金
国家自然科学基金(31371769)
广东省科技计划项目(2012A020100002)
广州市科技计划项目(2013J4100053)
大学生创新实验计划项目(L13186)
