摘要
为比较不同核酸提取方法检测禽流感病毒敏感性的差异，将 H5N1禽流感抗原做10－2-10－10稀释，分别用 Trizol 法、磁珠法和膜吸附法提取上述抗原稀释度核酸模板，采用荧光 RT-PCR 对其进行评价。结果表明，在不同稀释度的抗原的检测中，3种核酸提取方法以膜吸附法提取效率最高，其依次为磁珠法、Trizol 法，经方差分析，3种核酸提取方法之间存在显著性差异（P＜0．05）。说明 3种核酸提取方法提取效率存在显著差异，核酸提取效率的高低影响禽流感病毒荧光 RT-PCR 检测的敏感性，建议实验室根据实际情况选择核酸提取的方法。
To compare the efficiency of different nucleic acid extraction methods for detection of avian influ-enza virus.H5N1 avian influenza virus was used as template,Trizol,magnetic beads and membrane ad-sorption methods were used for nucleic acid extraction,H5N1 avian influenza virus were diluted from 10 -2 to 10-10 ,the efficiency of the three methods were evaluated through real-time RT-PCR.The variance anal-ysis showed there were significance differences among the three different methods（P〈0.05）.The mem-brane adsorption extraction method is the most efficient one,and followed by magnetic beads and Trizol extraction.The nucleic acid extraction efficiency is closely related to the sensitivity of the real-time RT-PCR results.The results suggested that the appropriate detection methods should be chosen for detection of clinical specimens based on actual situation.
出处
《动物医学进展》
CSCD
北大核心
2014年第11期116-119,共4页
Progress In Veterinary Medicine
关键词
禽流感病毒
核酸提取
提取效率
荧光RT-PCR
Avian influenza virus
nucleic acid extractions efficiency of extraction
real-time RT-PCR
