摘要
目的探讨拟除虫菊酯对大鼠甲状腺激素（TH）水平的影响。方法经口连续１５d给予大鼠氯菊酯（PM）１００、２００、４００mg·kg－１·d－１和溴氰菊酯（DM）６．２５、１２．５０、２５．００mg·kg－１·d－１。用放射免疫分析法检测大鼠血清、脑组织及其亚细胞结构中的TH水平。结果（１）PM：２００、４００mg·kg－１·d－１组大鼠血清三碘甲腺原氨酸（T３）、四碘甲腺原氨酸（T４）、游离T３（fT３）、游离T４（fT４）均下降，而促甲状腺激素（TSH）上升，且现PM呈现剂量依赖关系（T４：r＝０．６７９，fT４：r＝０．５１１，TSH：r＝０．７３８，均P＜０．０１）；１００mg·kg－１·d－１组大鼠T４和fT４下降，T３、fT３、TSH无明显变化。３个处理组大鼠大脑皮层T４分别为（３９．９±５．０）、（３３．４±９．２）、（３２．３±８．２）pg／mgpro，明显低于对照［（４４．２±７．１）g／mgpro］，且有剂量依赖性（r＝０．５２９，P＜０．０１）；而PM染毒４００mg·kg－１·d－１组的T３［（２１．２±５．２）pg／mgpro比对照［（３０．９±１０．０）pg／mgpro］明显降低。２００、４００mg·kg－１·d－１组大鼠海马T４为（１９．１±５．５）、（１９．２±６．５）pg／mgpro。
Objective To investigate the effects of pyrethroids on the concentrations of thyroid hormone in rat brain.Method Permethrin(PM)and deltamethrin(DM)were administered to the rats with daily doses of100,200and400mg ·kg -1 ·d -1 and6.25,12.5and25mg ·kg -1 ·d -1 ,respectivelly for15days.Serum and brain tissue determinations of thyroxin(T 4 )and triiodothyronine(T 3 )were performed by radioimmunoassay(RIA).Results PM induced a dose de-pendent decrease in the serum levels of T 4 ,T 3 ,fT 4 and fT 3 and an increase in the serum TSH levels,whereas DM was only able to induce a dose dependent decrease in the serum levels of T 4 .PM treatment reduced both the levels of T 4 and T 3 in homogenates of the cerebral cortex and hippocampus respectively,whereas the highest dose of DM decresed only the cerebral cortex levels of T 4 .The effects of subchronic treatment with PM and DM on the concentrations of T 3 were further investigated in the subcellular fractions,namely nuclei,mitochondria,myelin and synaptosomes of the cerebral cortex and hippocampus.PM treatment induced a decrease in the nuclear and synaptosomal concentrations of T 3 of either the cerebral cortex or hippocampus,whereas DM reduced the levels of T 3 especially in the mitochondria of the cortex and hippocampus.Conclusions Treatment with pyrethroids subchronically to the rats would affect the serum and brain tis-sue levels of T 4 and T 3 .These results indicate that the pyrethroids-induced neurotoxicity may involve at least in part an impairment of the physiological action of T 3 at its subcellular targets.[
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2002年第3期173-176,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
关键词
氯菊酯
溴氰菊酯
大鼠
血清
脑
甲状腺激素
Pyrethroids
Thyroid hormones
Neurotoxicity
Subcellular fractions
Rat brain
