摘要
目的 :探讨人参皂甙 (ginsenosides,Gin)单体Rgl及维拉帕米 (verapamil,Ver)对豚鼠心肌细胞内游离钙浓度 ([Ca2 + ]i)变化的影响。方法 :采用离体豚鼠心脏Langendorff法灌注 ,胶原酶I型分离心肌细胞 ,用荧光指示剂方法 (Fura 2 /AM)标记心肌 ([Ca2 + ]i)变化。将心肌细胞悬液分为 3组 :对照组、Rgl组和Ver组。观察缺氧后心肌[Ca2 + ]i 的变化。结果 :(1)正常氧状态心肌 [Ca2 + ]i 均值为 (12 5 .4± 10 .3)nmol/L(n =2 0 )。 (2 )缺氧状态下 ,心肌[Ca2 + ]i 增加与缺氧时间 (程度 )直线相关 ,相关系数r为 0 .98左右。 (3)Rgl对缺氧后心肌 [Ca2 + ]i 增加明显延缓。结论 :Rgl在缺氧条件下 ,使心肌 [Ca2 + ]i 明显下降 ,从而阻止心肌细胞内钙超载 ,其作用与Ver相似 。
Objective: Our aim was to study the effect of ginsenosides (Gin) and verapamil (Ver) on intracellular free calcium concentration of myocardial cell([Ca 2+ ] i) under hypoxic condition. Methods: We used guinea pig heart Langendorff instillation. The myocardial cells were isolated by collagenase (type I, Sigma) and marked by fluorescence ratio imaging .The suspension of myocardial cells was assigned to 3 groups: Rg1, Ver, and control. Each group was exposed to hypoxia and normoxic before determination of [Ca 2+ ] i. Results: In normoxic state,[Ca 2+ ] i was (125.4±10.3) nmol/L (n=20). In hypoxia state, the increased [Ca 2+ ] i of myocardial cells was proportional to the time (degree) of hypoxia. Correlation coefficient (r) was about 0.98. Under hypoxia, Rg1 obviously slowed the increase of [Ca 2+ ] i .Conclusion: In hypoxia Rg1 decreased the increased [Ca 2+ ] i. It can prevent intracellular calcium overload. The Rg1 may improve self protected performance of myocardial cells.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2001年第6期431-432,440,共3页
Journal of China Medical University
